【摘要】 目的观察缺氧诱导因子-1α(HIF-1α)对HepG2细胞生长及凋亡相关蛋白的影响,并探讨其意义。方法将H1F-1α转染入人肝癌细胞HepG2中,观察HepG2细胞生长。建立人肝癌裸鼠模型,随机将其分为两组：对照组(A组,10只),HIF-1α转染细胞组(B组,10只)。1个月后,切除瘤灶、称瘤重、计算促瘤率。标本用Western blot检测HIF-1α、凋亡相关蛋白Survivin、bcl-2和Caspase-3的表达。结果HIF-1α转染HepG2细胞后,细胞生长速率加快。转染HIF-α的裸鼠肿瘤生长较对照组快,A组、B组的瘤重分别为(3．51±0．33)、(4．64±0．40)g,促瘤率为32%。B组HIF-1α、Survivin和bcl-2的蛋白水平明显高于A组,但Caspase-3的水平则低于A组。结论转染HIF-1α体内、外均可促进肝癌HepG2细胞的生长,其机制除促进血管生成外,还可能与其能抑制凋亡有关。更多还原

【Abstract】 Objective To study the effects of hypoxia-inducible factor-lalpha(HIF-1α)on the expression of apoptosis related proteins and HepG2 cells growth.Methods To observe the growth rate of HepG2 cells after HIF-latransfected.HepG2 cells(1×10~6/mouse)were inoculated subcutaneously into 20 nude mice,which were randomly divided into two groups:the control group(group A,n=10),the HIF-1αtransfeeted group(group B,n=10).The weights of subcutaneous tumor were detected.The ex- pressions of HIF-1α,survivin,bcl-2 and caspase 3 were analyzed by Western blot.Results The growth rates of HIF-1αtransfected hepatic cancer cells were significantly increased,and more importantly,HIF- 1αwas able to enhance hepatic cancer growth in nude mice(P＜0.05).The expressions of HIF-1α、sur- vivin and bcl-2 in group B were increased significantly than that of group A,the expression of caspase 3 in group B was decreased significantly than that of group A.Conclusion HIF-1αcould increase hepatic can- cer cells HepG2 growth in vitro and in vivo and its mechanism may be due to the fact that It can inhibit apoptosis.更多还原