【摘要】 目的探讨γ射线消毒对不同保存方法牛颈静脉管道机械性能的影响,寻求牛颈静脉管道合适的灭菌和保存方法。方法：将采集、制作好的牛颈静脉拉力条按不同保存方法分为6组：新鲜对照组(A组),冷冻干燥组(B组),溶剂干燥组(C组),深冻组(D组),PBS缓冲液保存组(E组),60％乙醇保存组(F组)。各组经25 kGyγ射线消毒(A组不进行γ射线消毒)后分别行单轴拉伸试验、细菌培养及组织形态学观察。结果上述5种方法保存的牛颈静脉管道经γ射线消毒后,其最大负荷与极限抗张强度分别为(25．23±15．95)N、(4．6±2．53)Mpa(B组),(35．28±14．66) N、(7．10±2．08)Mpa(C组),(25．44±7．97)N、(5．20±1．43)Mpa(D组),(31．55±12．32)N、(6．20±2．72)Mpa(E组),(32．02±18．44)N、(6．80±2．03)Mpa(F组)。与对照组[(23．43±11．21)N、(5．12±3．06)Mpa]相比,P＞0．05。断裂延伸率分别为(86．08±31．21)％(B组)、(137．99±38-80)％(C组)、(133．39±42．19)％(D组)、(153．23±55．00)％(E组)和(142．09±54．03)％(F组),与对照组(193.80±37．53)％相比,P＜0．05。细菌培养结果全部为阴性。组织切片显示B组和D组组织呈空腔空泡状改变。结论经25 kGyγ射线消毒后,上述五种保存方法处理牛颈静脉管道其最大负荷与极限抗张强度变化不明显,但其断裂延伸率均下降,尤以冷冻干燥保存组为甚。更多还原

【Abstract】 Objective To explore the effect of gamma irradiation sterilization on biomechanical properties of bovine jugular vein conduit (BJVC) under different preservation methods and to find out the suitable methods of s(?)erilization and preservation of BJVC.Methods The strips of BJVC were divided into 6 groups according to the different preservation methods:fresh control group (group A),freeze-dried group (group B),solvent-dehydrated group (group C),deep-frozen group (group D),preservation with PBS buffer group (group E),preservation with 60% alcohol group (group F).All groups underwent uni- axial load testing,bacteria culture and histomorphology observation after being sterilized by gamma irradi- ation (25 KGy) (gamma irradiation sterilization were not carried out in group A).Results The maxi- mum load and ultimate tensile strength of strips were (25.23±15.95) N,(4.60±2.53) Mpa (group B),(35.28±14.66) N,(7.10±2.08) Mpa (group C),(25.44±7.97) N,(5.20±1.43) Mpa (group D),(31.55±12.32) N,(6.2±2.72) Mpa (group E),and (32.02±18.44) N,(6.8±2.03) Mpa (group F) respectively,not statistically different from those in group A [(23.43±11.21) N,(5.12±3.06) Mpa,P＞0.05].The elongation rate of strips in groups B,C,D,E and F was (86.08±31.21)%,(137.99±38.80)%,(133.39±42.19)%,(153.23±55.00)% and and (142.09±54.03)% respectively,significantly lower than in group A [(193.80±37.53)%,P＜0.05].The result of bacteria culture of each sample was negative.Tissue paraffin-embedded sections showed that the tissues in groups B and D had cavitary and vacuohis changes.Conclusion The maximum load and ultimate ten- sile strength of BJVC have not obvious change after being sterilized by gamma irradiation (25 KGy), while their elongation rate is decreased,specially in freeze-dried group.更多还原