【摘要】 目的研究骨桥蛋白(OPN)促进肝癌转移的可能机制。方法经质粒转染、G418筛选获得稳定高表达OPN人肝癌细胞株SMMC-7721,以空质粒转染组为对照。比较两组细胞的肿瘤转移相关基因表达差异。选取其中3个肿瘤转移相关基因(MMP-2、MMP-9、uPA),用ELISA方法检测其细胞培养上清液的蛋白表达情况。结果经肿瘤转移基因芯片比较发现SMMC-7721高表达OPN后,有88个肿瘤转移相关基因表达上调,有6个肿瘤转移相关基因表达下调。高表达OPN SMMC- 7721上清液MMP-2、uPA水平明显高于对照组[(46．11±3．12)vs(19．66±3．69)μg/L,(5．36±0．94)vs(1．39±0．64)μg/L,而MMP-9表达水平差异无统计学意义[(7．97±2．42)vs(7．25±2．14)μg/L,]与基因芯片结果符合。结论在SMMC-7721肝癌细胞株中OPN高表达引起多个肿瘤转移相关基因改变。OPN可能通过上调MMP-2、uPA等基因表达促进肝癌转移。更多还原

【Abstract】 Objective To study the possible mechanism of osteopontin (OPN) promoting hepato- cellular carcinoma metastasis.Methods Human hepatocellular carcinoma cell line SMMC-7721 stably ex- pressing high level of OPN was established by plasmid transfection and G418 screening,while cells trans- fected with mock plasmid served control group.The expression differences of metastasis-related genes be- tween two groups were compared by gene chips.Concentrations of MMP-2,MMP-9 and uPA in the medium between them were measured by ELISA.Results Eighty-eight metastasis-related genes of SMMC-7721 highly expressing OPN were up-regulated,while 6 genes were down-regulated.MMP-2 and uPA concentrations in the medium of SMMC-7721 expressing OPN were higher than in control group [(46.11±3.12) vs (19.66±3.69)μg/L,(5.36±0.94) vs (1.39±0.64)μg/L respectively],but MMP-9 concentration was not increased [ (7.97±2.42) vs (7.25±2.14)μg/L].Conclusion OPN causes expression changes in many metastasis-related genes of SMMC-7721.OPN might enhance the ex- pression levels of MMP-2,uPA and other genes to facilitate hepatocellular carcinoma metastasis.更多还原