【摘要】 目的观察bcl-2、bax mRNA在三氧化二砷（As₂O₃）诱导人类胆管癌株QBC939、RBE细胞凋亡中的表达。方法Rhodamine123染色检测线粒体膜通透性变化；逆转录一聚合酶链反应（RT- PCR）检测bcl-2、bax mRNA的表达。结果As₂O₃干预,实验组Rhodamine123荧光染色强度较对照组明显减弱；凝胶成像检测bcl-2/β-actin mRNA A比值,QBC939、RBE细胞对照组分别为0．41±0．03、0．84±0．03；24 h实验组为0．01±0．01、0．43±0．02。hax/GAPDH mRNA A比值QBC939、RBE细胞对照组分别为0．21±0．01、0．42±0．04；24 h实验组为1．44±0．16、1．15±0．21,对照组与实验组比较差异均有统计学意义（P＜0．01）。结论As₂O₃可能增加胆管癌细胞株线粒体膜的通透性,下调bcl-2 mRNA表达,上调bax mRNA的表达,可能启动了线粒体凋亡信号传导途径。更多还原

【Abstract】 Objective To research the expression of bcl-2/bax mRNA in As₂O₃ induced cholan- giocarcinoma cells line（QBC939,RBE） apoptosis in vitro.Methods The mitochondria permeability was measured by Rhodamine123 assay;The bcl-2/bax mRNA expression was detected by RT-PCR.Results The Rhodamine123 fluorescein stain intensity in the experiment group after treatment with As₂O₃ was decreased as compared with that in the control group.Gel imaging system revealed that the bcl-2/β-actin mRNA optical density ratio of QBC939/RBE cells in control group was 0.41±0.03/0.84±0.03 and 0.01±0.01/0.43±0.02 in experiment group respectively,and the bax/GAPDH mRNA optical density ratio of QBC939/RBE cells in control group was 0.21±0.01/0.42±0.04 and 1.44±0.16/1.15±0.21 in experiment group respectively.There was significant difference between control group and experi- ment group （P＜0.01）. Conclusion As₂O₃ may induce mitochondrial membrane depolarization,down- regulate the expression of bcl-2 mRNA and up-regulate the expression of bax mRNA expression in cholangiocarcinoma cells line.As₂O₃ may activate the mitochondria apoptosis signal transduction pathway.更多还原