【摘要】 目的探讨膀胱癌中上皮钙黏附素（E-cd）和树突细胞（DC）的表达及其与生物学行为的关系。方法免疫组织化学检测采用链霉素-生物素（LSAB）免疫酶染色法,对65例膀胱癌手术切除标本中的E-cd、DC的表达进行了对照检测,染色时用已知E-cd和S100阳性的正常膀胱黏膜及膀胱肌层内神经作阳性对照,用PBS取代一抗作阴性对照。结果65例膀胱癌中33例E-cd正常表达（50．8%）,20例DC正常表达（30．8%）,E-cd正常表达率在Ⅰ、Ⅱ和Ⅲ级肿瘤中分别为70．1%、66．6%和25．9%,在T_is-1期和T_2-4期肿瘤中分别为80．6%和23．5%；DC正常表达率在Ⅰ、Ⅱ和Ⅲ级肿瘤中分别为76．5%、33．3%和0,在T_is-l期和T_2-4期肿瘤中分别为63．3%和2．7%。在不同病理分级和临床分期的膀胱癌中,E-cd和DC的正常表达率差异有统计学意义（P＜0．05）。结论E-cd和DC的正常表达率均随膀胱癌的病理分级、临床分期的上升而下降,E-cd和DC正常表达者的肿瘤复发率和死亡率均显著低于异常表达表。E-cd和DC可作为预测膀胱癌生物学行为的指标之一。更多还原

【Abstract】 Objective To investigate the expression of dendritic cells（CDs）and E-cadherin（E- cd）in bladder cancer and its biological significance.Methods By using immunohistochemical method and the LSAB immune enzyme staining method,the expression of E-cd and DCs was detected in the surgically resected specimens of 65 cases of bladder carcinomas.When staining,the normal bladder mucosa and blad- der muscular nerve positive for known E-cd and S100 served as positive controls and negative controls were designated by using PBS instead of primary antibody.Results The positive expression rate of E-cd was 50.8% in bladder cancer and that of DCs was 30.8%.The positive expression rate of E-cd in gradeⅠ,ⅡandⅢtumors was 70.1%,66.6% and 25.9% respectively and that in stage T_is-T₁ and T₂-T₄ tumors 80.6% and 23.5% respectively.The positive expression rate of DCs in gradeⅠ,ⅡandⅢtu- mors was 76.5%,33.3% and 0 respectively and that in stage T_is-T₁ and T₂-T₄ tumors 63.3% and 2.7% respectively.The positive expression rate of E-cd and DCs was significantly lower in poorly differ- entiated and invasive bladder cancers.Conclusion The decrased E-cd and DCs expression could be used as tumor markers for bladder cancer.更多还原