【摘要】 目的探讨大黄素防止肝纤维化的作用。方法用SD大鼠120只随机分为：(1)假手术+生理盐水组(SO+NS组)；(2)单纯胆总管结扎+生理盐水组(BDL+NS组)；(3)胆总管结扎+大黄素组(BDL+ED组),每组40只。BDL+NS组作胆总管结扎、生理盐水灌胃；BDL+ED组行胆总管结扎、大黄素混悬液灌胃。分别于14、21 d各处死每组大鼠的50%,观察肝纤维化情况和检测肝功能、小肠细菌移位。结果结扎胆总管大鼠的肠黏膜、肝纤维化及肝功能的受损随结扎时间延长而逐渐加重,但用大黄素组有显著减轻,结扎后21 d BDL+NS组与BDL+ED组比较,细菌移位率为(85%比50%,P＜0．05)；肝纤维化程度(按0～4级计)为[0、4、6、8、2比4、10、3、3、0只,(P＜0．01)]；肝功能：ALT[(212．4±19．2)U/L比(146．5±16．2)U/L(P＜0．01)]；AST [(709．6±39．9)U/L比(612．2±30．8)U/L(P＜0．05)]；TB[(174．4±32．2)μmol/L比(159．8±27．2)μmol/L P＜0．05)]。结论大黄素能降低阻塞性黄疸时的肠道细菌移位率,对肝功能有明显保护作用,可有效减轻梗阻性黄疸时的肝纤维化。更多还原

【Abstract】 Objective To observe the preventive effect of emodin on the liver fibrosis(cirrhosis). Methods 120 SD rats were randomly divided into three groups:the sham operation group(SO+NS group),choledochus ligature+normal saline group(BDL+NS group)and choledochus ligature+emodin group(BDL+ED group,40 in each group.Each group was randomly divided into two subgroups:14-day group and 21-day group,each having 20 rats.After 14 and 21 days,rats were sacrificed.Plasm levels of ALT,AST and TB were determined,and bacterial translocation rate and degree of liver fibrosis were eval- uated too.Results On the 21st day after experiments,bacterial translocation rate was obviously higher in BDL+NS group than in BDL+ED group(85% vs 50%,P＜0.05);liver fibrosis(by 0-4 degrees)was more severe in BDL+NS group than in BDL+ED group(0,4,6,8,2 vs 4,10,3,3,0 rats,P＜0.01); plasm ALT,AST and TB wereremarkably higher in BDL+NS group than in BDL+ED group(ALT: 212.4±19.2 vs 146.5±16.2,P＜0.01;AST:709.6±39.9 vs612.2±30.8,P＜0.05;TB:174.4±32.2 vs 159.8±27.2,P＜0.05).Conclusion Emodin can obviously decrease the degrees of liver fibro- sis,decline the plasm levels of ALT,AST and TB and bacterial translocation.更多还原