【摘要】 目的研究提高犬胰岛体外培养质量的方法。方法将犬胰岛分别进行普通培养 (A组)、常温(37℃)微重力培养(B组)和低温(26℃)微重力培养(C组),分别培养3、7、14和21 d, 并检测各组胰岛培养后收获率、存活率和功能。结果不同方法培养21 d后,B组和C组平均胰岛收获率为(66．2±4．1)％和(81．3±4．8)％高于A组的胰岛收获率(50．5±2．9)％,P<0．05。A组胰岛存活率降至(27．7±3．5)％,与B组的(49．7±3．2)％和C组的(64．8±4．7)％比较,P<0．05。 B组和C组DNA含量及胰岛素含量均维持较高的水平,B组和C组胰岛素储备能力较A组高,胰岛索刺激指数分别为(3．30±0．70)、(5．48±1．00)和(2．33±0．50),差异有统计学意义(P< 0．05)。结论低温微重力三维培养胰岛是提高胰岛培养质量的好方法。更多还原

【Abstract】 Objective To find and develop a reliable improved method for culture of islets in canine. Methods The purified canine islets were divided into 3 groups:Group C:The islets were cultured in HRCCS for 3,7,14 and 21 days;Group B:Fresh islets cultured in RCCS;Gorup A:Canine islets were assigned to flask-culture in common medium for the same time as group C. Survival islets of the canine in 3 groups were measured by AO-PI and dithizone (DTZ), and insulin contents in different culture liquids were determined by radioimmunoassay. Results When islet cells were cultured for 3,7,14 and 21 days, survival rate of HRCCS-cultured islets was (90.6± 3.9)%, (83.8± 3.8)%, (73.9± 4.3)% and (64.8±4.7)% higher than RCCS-cultured islets and flask-cultured islets (P<0.05). The insulin release and insulin stimulation index in islets incubated in HRCCS (5.48 ± 1.00) were higher than those in RCCS-culture (3.3010.70) and common flask-culture (2.33 ±0.50), P<0.05. HRCCS-cultured islet recovery rate was (81.3±4.8)% higher than RCCS-cultured islets (66.2±4.1)% and flask-cultured islets (50.5± 2.9)% on the cultured 21 days (P< 0.05). Conclusion HRCCS are in favor of the growth and proliferation of islets, and the islets cultured in HRCCS have better insulin secreting capacity.更多还原