节点文献
血管内皮生长因子D基因转移对胆汁性肝硬变大鼠肝纤维化和门静脉压力的影响
The effect on liver fibrosis and portal pressure after administration of expression vector encoding for endothelial growth factor D in biliary cirrhotic rats
【摘要】 目的观察胆汁性肝硬变大鼠门静脉注射血管内皮生长因子D(hVEGF-D)后的促血管形成效应以及对肝纤维化和门静脉压力的影响。方法30只SD大鼠胆总管结扎法制作胆汁性肝硬变模型,治疗组门静脉注射PCHO/hVEGF-D 150μg/只,2周后比较肝组织纤维化程度(苏木素-伊红染色、浸银染色法)、门静脉压力、VEGF蛋白质表达、肝组织微血管密度改变。结果肝组织纤维化程度较注射前明显降低;门静脉压力治疗前为(15.45±1.97)cm H2O(1 cmH2O= 0.098 kPa);治疗后则变为(12.56±1.86),差异有统计学意义(P<0.05)。治疗组VEGF蛋白质表达平均染色积分为6.56±1.81,肝硬变对照组4.4±1.02,差异有统计学意义(P<0.01)。治疗组血管计数为14.33±3.24;肝硬变对照为9.2±1.48(P<0.01)。结论胆汁性肝硬变大鼠门静脉注射血管内皮生长因子D表达载体后可以一定程度上促进肝内血管形成、减缓肝纤维化程度降低门静脉压力。
【Abstract】 To investigate the expression efficiency and its therapeutic value of plasmid vector encoding for vascular endothelial growth factor D (PCHO/hVEGF-D) after intravenous injection to cirrhotic rat liver due to cholestasis. Methods The model of biliary cirrhosis was established in 30 rats by ligation of common bile duct for 4 weeks. The pressure of portal vein, fibrosis change in liver, VEGF protein expression and vascular density were compared before and 2 weeks after administration of PCHO/ hVEGF-D 150 mg for each. VEGF protein was identified by histoimmunochemistry and vascular endothelial cells were marked with Factor Ⅷ. Results The average pressure of portal vein before treatment was 15.45± 1.97 cm H2O, and decreased to 12.56±1.86 with significant difference after treatment with PCHO/hVEGF-D (P<0.05). The fibrosis degree was also relieved remarkably. The VEGF protein expression was elevated to 6.56 + 1.81 while only 4.4 ± 1.02 in control group (P < 0.01). The average number of capillaries was statistically more in treatment group than in control group (14.33± 3.24 vs 9.2±1.48,P<0.01).Conclusion Venous injection with PCHO/HVEGF-D to rat could induce angio-genesis as a result of high expression of VEGF. Liver fibrosis and portal pressure could be relieved after angiogenesis in cirrhotic rats due to cholestasis.
【Key words】 Liver cirrhosis; Angiogenesis; Gene therapy; Endothelial growth factor;
- 【文献出处】 中华实验外科杂志 ,Chinese Journal of Experimental Surgery , 编辑部邮箱 ,2006年03期
- 【分类号】R575.2
- 【被引频次】3
- 【下载频次】66