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蛋白酶体亚基RC2在失神经骨骼肌中的变化规律及其意义

Expression pattern and significance of proteasome subunit RC2 in rat denervated skeletal muscles

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【作者】 冯勇张高孟马学晓李继峰顾玉东

【Author】 FENG Yong ZHANG Gao-meng MA Xue-xiao Department of Hand Surgery,Huashan Hospital,Fudan University,Shanghai 200040,China

【机构】 复旦大学附属华山医院手外科复旦大学附属华山医院手外科

【摘要】 目的研究蛋白酶体亚基RC2在失神经骨骼肌中的变化规律及其意义。方法将48只SD大鼠,建立右下肢腓肠肌失神经支配模型,随机分为8组,每组6只,术后0 d为对照组,术后1、2、3、4、5、6、14 d为实验组;分别采用实时定量PCR和Western blot检测RC2在转录和蛋白水平的变化规律。结果RC2的mRNA从失神经后第1天开始表达上调,3 d达到高峰,为对照组的4.98倍,随后逐渐下降,14 d时降为对照组的0.42倍;各实验组与对照组相比,差异有统计学意义(P<0.05)。RC2在蛋白水平的变化与转录水平有着相同的趋势,但是变化幅度较转录水平为低。失神经后3 d达到高峰,为对照组的2.70倍,随后缓慢下降,14 d时降为正常侧的O.35倍;失神经术后1、6 d与对照组相比,差异无统计学意义(P>0.05)。2~5、14 d与对照组相比,差异有统计学意义(P<0.05)。结论蛋白酶体亚基RC2在失神经骨骼肌早期表达增强,提示泛素-蛋白酶体途径在失神经骨骼肌早期的蛋白快速降解中占有重要地位。

【Abstract】 Objective To observe expression of proteasome subunit RC2 in denervated skeletal muscles,and investigate the molecular mechanism of skeletal muscle wasting.Methods A model of gastrocnemius muscle denervation at the right lower limb was established in 48 rats.They were divided into 8 groups with 6 each.The subsequent changes in RC2 mRNA and protein levels were determined by real-time quantitative PCR and Western blot, respectively.Results Expression of proteasome subunit RC2 mRNA in rat denervated gastrocnemius muscle was upregulated gradually,peaked in 3 days,then decreased (4.98 folds and 0.42 fold at day 3 and day 14,respectively; P<0.05).The changes of protein levels were similar to that of mRNA levels,but the amplitude of changes was lower (2.70 folds and 0.35 fold at day 3 and day 14,respectively;P<0.05).Conclusion After denervation, expression of proteasome subunit RC2 in skeletal muscle was upregulated.This supports the concept that denervation- induced muscle atrophy is,at least in part,regulated by the ubiquitin-proteasome proteolytic pathway.

【关键词】 肌萎缩肌,骨骼蛋白酶体去神经支配
【Key words】 Muscle atrophyMuscleskeletalProteasomeDenervation
【基金】 国家自然科学基金(30371463)
  • 【文献出处】 中华手外科杂志 ,Chinese Journal of Hand Surgery , 编辑部邮箱 ,2006年02期
  • 【分类号】R746.4
  • 【被引频次】6
  • 【下载频次】51
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