【摘要】 目的观察柑皮提取物对人增生性瘢痕成纤维细胞增殖以及胶原代谢的影响。方法人增生性瘢痕皮肤标本取自笔者单位行整形术的2例烧伤患者。采用组织块法培养成纤维细胞后分为:实验组,细胞加入成纤维细胞培养基与柑皮提取物,并根据提取物的浓度分为2．5、5．0、10．0、25．0 mg／L4个部分;空白对照组,细胞仅加入成纤维细胞培养基;对照组,细胞加入体积分数5％乙醇与成纤维细胞培养基。采用噻唑蓝比色分析法、原位缺口末端标记法及放射免疫法观察各组成纤维细胞增殖情况,计算增殖抑制率;计算凋亡指数(AI)以及检测Ⅰ型胶原交联羧基末端肽(ICTP)、Ⅰ型前胶原氨基端前肽(PINP)含量的变化。结果实验组2．5-25．0 mg／L柑皮提取物的细胞增殖抑制率分别为(7．100±0．038)％、(8．100±0．048)％、(10．900±0．055)％、(15．900±0．097)％; AI分别为69．7％、71．7％、86．4％、95．2％;ICTP分别为(17．2±0．6)、(18．3±0．6)、(19．8±0．5)、(23．2±0．6)μg／L;PINP分另0为(101．7±1．4)、(107．8±1．1)、(111．6±1．2)、(124．6±1．3)μg／L,均明显高于对照组(P<0．05)。空白对照组上述指标与对照组相近(P>0．05)。结论柑皮提取物能抑制人增生性瘢痕成纤维细胞增殖,促进其凋亡及Ⅰ型胶原分解,具有治疗和预防增生性瘢痕的作用。更多还原

【Abstract】 Objective To investigate the effects of citrus reticulata bianco extract on the proliferation and collagen metabolism of fibroblasts from human hypertrophic scar. Methods Human hypertrophic scar fibroblasts from two burn patients obtained from plastic surgery were cultured in vitro and divided into experimental group (n = 12, with basic culture medium and 2. 5 ,5.0,10.0,25. 0 mg/L citrus reticulata bianco extract, respectively, 3 bottles for each concentration of citrus reticulata bianco extract) , control group 1(n = 3, with basic culture medium) , and control group 2(n =3, with basic culture medium and 5% ethyl alcohol). The cell proliferation in each group was observed with MTT method, then the inhibition rate was calculated. Apoptosis and its index(AI) in each group were determined after TUNEL staining . The changes in the content of ICTP and PINP in each group were observed by radioimmunity. Results The inhibition rate in the experimental group with the citrus reticulata bianco extract in concentration of 2.5, 5.0, 10.0,25. 0μg/ ml were (7. 100±0. 038) % , (8. 100±0.048)% ,(10.900±0.055)% ,(15.900±0.097)%,respectively, which were significantly higher than those in other two groups ( P <0.05 ). The AI (69. 7% , 71.7% , 86.4% ,95.2%), ICTP [(17.2±0.6), (18.3±0.6), (19.8±0.5), (23.2±0.6)μg/L] and PINP [(101.7±1.4) , (107.8±1.1) , (111.6±1.2) , (124.6±1.3)μg/L] in experimental group with the citrus reticulata bianco extract in concentration of 2.5, 5.0, 10.0 ,25.0 mg/L were also obviously higher than other two control groups(P <0.05) ,but these indices in control 1 group were similar to those in control 2 group(P>0.05). Conclusion The citrus reticulata bianco extract might be beneficial for the management of hypertrophic scar through inhibition of the proliferation of fibroblasts in hypertrophic scar, by promoting apoptosis and collagen degradation.更多还原