【摘要】 目的探讨JAK/STAT通路中信号转导和转录激活因子Stat 1和含SH2区域的酪氨酸磷酸酶SHP-1在尖锐湿疣中的异常表达。方法原位杂交技术诊断HPV6/11相关尖锐湿疣,SP免疫组化染色技术检测40例HPV6/11阳性尖锐湿疣患者皮损、20例正常人皮肤(包皮)中Stat 1和SHP-1的表达及分布。结果尖锐湿疣皮损中Stat 1和SHP-1的表达均强于正常人上皮中的表达,两者比较差异均有统计学意义(前者W=382．5,P＜0．01；后者W=369,P＜0．01)。尖锐湿疣患者皮损中Stat 1和SHP-1阳性细胞主要分布于棘细胞层,Stat 1阳性反应定位于胞浆或胞核,SHP-1阳性反应主要定位于胞浆。尖锐湿疣患者中Stat 1和SHP-1的表达呈显著相关性(tau-b=1．0,P＜0．01)。结论HPV6/11阳性尖锐湿疣中Stat 1和SHP-1表达增强,提示HPV6/11感染时可能通过JAK/STAT通路,在角质形成细胞过度增殖中起作用。更多还原

【Abstract】 Objective To investigate the abnormal expression of signal transducers and activators of transcription 1(Stat 1)and Src-homology 2 domain phosphatasel(SHP-1)in JAK/STAT route of condyloma acuminatum(CA)and its significance.MethodsHPV6/11 related CA was diagnosed by in situ hybridization;the expression and distribution of Stat 1 and SHP-lwere examined by SP immunohisto- chemistry in 40 specimens from CA patients positive for HPV 6/11 and 20 normal skin specimens (fore- skins).ResultsThe expression of both Stat 1 and SHP-1 was significantly higher(both P＜0.01) in CA lesions than that in normal epithelium.The Stat 1-and SHP-1-positive cells were mainly distributed in prick- le layer of HPV 6/11 related CA lesions.The positive signal of Stat 1 was located in the cytoplasm or nucle- i,and that of SHP-1 was mainly distributed in the cytoplasm.There was a significant correlation(P＜0.01)between the expression of Star 1 and that of SHP-1 in HPV 6/11-related CA lesions.Conclusion The expression of Stat 1 and SHP-1 is increased in HPV6/11 positive CA lesions,which suggests that HPV6/11 may play a role in the over-proliferation of keratinocytes through JAK/STAT route.更多还原