【摘要】 目的探讨湘籍汉族特应性皮炎患者IgE高亲和力受体β链(FcεRIβ)基因启动子-109位基因多态性及与血清总IgE的关系。方法采用PCR-单链构型多态性(SSCP),DNA测序分析FcεRIβ基因多态性,ELISA检测血清总IgE。结果20例特应性皮炎患者中有4例FcεRIβ基因启动子-109位泳动异常,其中有2例特应性皮炎患者第138位碱基发生G→A替换,159位碱基发生T→C替换；2例患者165位碱基发生A→G替换。突变组特应性皮炎患者血清总的IgE为(1．88±1．30)mg/L,无突变组特应性皮炎患者血清总的IgE为(0．32±0．12)mg/L,正常人对照组血清总的IgE为(0．20±0．05)mg/L；突变组较无突变组及正常人对照组差异有统计学意义(P＜0．05)。结论特应性皮炎患者IgE高亲和力受体β链启动区-109位多态性伴有IgE高表达。更多还原

【Abstract】 Objective To investigate the-109 polymorphism at the promoter region of high-affinity IgE receptorβ(FceR1β)gene and its relationship with serum IgE in patients with atopic dermatitis in Han nationality in Hunan Province,China.MethodsDNA was obtained from 20 patients with atopic der- matitis and 20 healthy controls.The-109 polymorphism was detected by PCR-single strand conformation polymorphism (SSCP),and confirmed by direct sequencing.ELISA was applied to measure the serum total IgE.Results Three mutations were identified in 4 of the 20 patients,including a G138A mutation and a T159C mutation in 2 patients,as well as a A165G mutation in the other 2 patients.The level of serum total IgE was 1.88±1.30 mg/L in the 4 patients with mutations,which was significantly different(P＜0.05) from that (0.32±0.12 mg/L)in the 16 patients without mutations and that(0.20±0.05 mg/L)in the healthy controls.ConclusionThe-109 polymorphism at the promoter region of FceRIβgene may be as- sociated with the increase of serum IgE level.更多还原