【摘要】 建立了HPLC法测定生物样品中间尼索地平的含量,并研究了其在大鼠体内的组织分布、排泄及血浆蛋白结合率等特征。以尼莫地平为内标,样品采用液-液萃取。采用C18柱,流动相为乙腈-20mmol/LKH2PO4溶液(60∶40),检测波长237nm。间尼索地平在2～1000ng/ml范围内,线性关系良好。间尼索地平在大鼠体内分布广泛,经粪便及胆汁的排泄量均较少,而尿中则未检出药物原型,间尼索地平的血浆蛋白结合率达97%以上。更多还原

【Abstract】 A HPLC method was established for determination of m-nisoldipine in rats. The tissue distribution and excretion of m-nisoldipine in rats and its plasma protein binding were discussed. Nimodipine was used as internal standard. The samples were extracted by liquid-liquid extraction method. A C18 column was used with the mobile phase of acetonitrile-20mmol/L KH2PO4 solution (60∶40), at the detection wavelength of 237 nm. The calibration curves of m-nisoldipine were linear in the range of 2 - 1000 ng/ml. m-Nisoldipine could be detected in all tissue samples but not been found in urine. The excretions through the faeces and bile were low. Plasma protein binding ratio of m-nisoldipine was over 97%.更多还原