【摘要】 目的探讨髓细胞白血病基因1(MCL-1)、Survivin在B细胞非霍奇金淋巴瘤(B-NHL)表达及其与细胞凋亡的关系。方法应用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术和免疫组织化学SP法检测43例B-NHL和10例反应性增生(RH)淋巴组织细胞凋亡率和MCL-1、Survivin的表达水平。结果MCL-1和Survivin在43例B-NHL中阳性表达率分别为58·1%(25/43)和69·8%(30/43),而MCL-1在RH淋巴组织阴性表达,Survivin在RH淋巴组织低表达10·0%(1/10),MCL-1在侵袭性B-NHL表达的频率和强度明显高于惰性B-NHL(70·0%,30·8%,P<0·05),Survivin在侵袭性B-NHL中表达的阳性率明显高于惰性B-NHL(80·0%,46·2%,P<0·05);MCL-1阳性表达组与凋亡指数(AI)无相关,Survivin阳性表达组与AI正相关(r=0·429,P<0·01),此外,两者表达呈显著正相关(r=0·598,P<0·001)。结论MCL-1对B-NHL细胞凋亡作用不如Survivin明显,后者可能参与了B-NHL细胞凋亡调节,二者在B-NHL的共表达与其发生发展有关。更多还原

【Abstract】 Objective To explore the expression of myeloid cell leukemia 1 (MCL-1) proteins and survivin and its correlation with cell apoptosis as well as with the development and progression of B cell non-Hodgkin′s lymphoma (B-NHL). Methods TdT-mediated dUTP nick end labeling and immunohistochemistry were used to study cell apoptosis and expression of MCL-1 proteins and survivin proteins in 43 patients with B-NHL and 10 with reactive hyperplasia (RH) lymphoid tissue. Results The positive rate of MCL-1 proteins and survivin proteins was 58.1% (25/43) and 69.8% (30/43) respectively. The expression of MCL-1 proteins was not detected in RH lymphoid tissue, but that of survivin was detected in 10.0% (1/10). The expression of MCL-1 proteins in aggressive B-NHL was higher than that in indolent B-NHL (70.0 % vs 30.8 %, P<0.05). The expression of survivin in aggressive B-NHL was also higher than that in indolent B-NHL (80.0% vs 46.2%,P<0.05). Apoptotic index (AI) was not correlated positively with the expression of MCL-1,but correlated positively with the expression of survivin (r=0.429,P<0.01). MCL-1 and survivin were correlated positively in B-NHL(r=0.598,P<0.001). Conclusions MCL-1 proteins as family member of BCL-2 have no influence on apoptosis but survivin may participate in the regulation mechanism of B-NHL apoptosis. It is indicated that the two proteins with a close relationship may take part in the development and progression of B-NHL.更多还原