【摘要】 目的探讨CD4+CD2+5调节性T细胞(CD4+CD2+5TREG细胞)在持续性HCV感染患者CD4+T细胞下调中的意义。方法流式细胞术检测慢性丙型肝炎患者外周血中CD4+CD2+5TREG细胞的数量以及细胞内因子的合成;与正常人或患者CD4+CD25-T细胞共同培养,检测其抑制功能;RT-PCR检测FOXP3的MRNA表达。结果CD4+CD2+5TREG细胞约占慢性丙型肝炎患者外周血中CD4+T细胞的(13.5±1.8)%,高于正常对照(5.3±0.8)%(P=0.004);主要合成IL-10,高表达FOXP3;CD4+CD25+TREG细胞显著抑制CD4+T细胞的增殖,以及合成IFNΓ,并且抑制活性较正常人增高(P=0.034),这种作用不依赖IL-10和转化生长因子Β。结论持续性HCV感染患者CD4+CD2+5TREG细胞表达增加,抑制活性增强,特异性抑制TH1反应。更多还原

【Abstract】 Objective To study the role of CD⁺₄ CD⁺₂₅regulatory T cells(CD⁺₄ CD⁺₂₅Treg cells) in CD⁺₄T cell responses in patients with persistent infection of hepatitis C viruses.Methods Flow cytometry was used to determine the number of CD⁺₄ CD⁺₂₅Treg cells and intracellular cytokine production by CD⁺₄ CD⁺₂₅Treg cells in patients with chronic HCV infection.To assess their regulatory properties CD⁺₄ CD⁺₂₅Treg cells were co-cultured with CD⁺₄ CD^-₂₅ T cells from patients or controls;the expressions of Foxp3 were measured by RT-PCR.Results CD⁺₄ CD⁺₂₅Treg cells comprised（13.5± 1.8）% of peripheral CD⁺₄T cells in the blood of persistent hepatitis C virus infected patients,which was significantly higher than that of healthy controls（5.3±0.8）%（P=0.004）.CD⁺₄ CD⁺₂₅Treg cells highly expressed Foxp3 and mainly synthesized IL-10;CD⁺₄ CD⁺₂₅Treg cells dramatically suppressed the proliferation of CD⁺₄ T cells and the production of IFN γ;the suppressive activity of CD⁺₄ CD⁺₂₅Treg cells in patients with persistent HCV-infection was higher than that in healthy controls（P=0.034）.These effects were dose-dependent but IL-10 and transforming growth factor β independent.Conclusion Patients with persistent hepatitis C virus infection show an increased number and suppressive activity of CD⁺₄ CD⁺₂₅Treg cells,which could function in a highly regulatory capacity to suppress Th1 response.更多还原