【摘要】 目的探讨凋亡抑制基因Livin表达在膀胱癌(TCC)发生发展中的作用。方法采用RT-PCR方法检测36例TCC组织标本中Livin的表达。男31例,女5例。年龄26~82岁,平均64岁。临床分期T122例,T29例,T33例,T42例。病理分级Ⅰ级10例,Ⅱ级23例,Ⅲ级3例。初发25例,复发11例。单发19例,多发17例。12例正常膀胱组织标本作对照。结果36例TCC中Livin阳性表达28例(78%),T1表达率82%(18/22),T2~T471%(10/14);Ⅰ级表达率70%(7/10),Ⅱ级83%(19/23),Ⅲ级67%(2/3);单发表达率79%(15/19),多发者77%(13/17);初发者80%(20/25),复发者73%(8/11)。Livin表达与TCC临床分期和病理分级不相关,与肿瘤数量,复发次数不相关(P>0.05)。对照组12例标本中均不表达。TCC组和对照组Livin表达差异有统计学意义。结论Livin表达与TCC的发生有关,可作为TCC的分子标志物,可能成为TCC诱导凋亡治疗的新靶点。更多还原

【Abstract】 Objective To study the expression of Livin, an apoptosis inhibitor gene,in tissues of transitional cell carcinoma of bladder and to investigate its clinical and pathological implications. Methods RT-PCR was used to determine the expression of Livin in 36 cases （31 men and 12 women） of TCC and 12 cases of normal bladder tissues （controls）.The mean age of the 36 cases at diagnosis was 64 years.The pathological grading were GradeⅠin 10 cases, GradeⅡin 23 cases,and Grade Ⅲ in 3 cases.The clinical staging were T₁ in 22 cases,T₂ in 9 cases,T₃ in 3cases,T₄ in 2 cases.Of the 36 cases,25 primary and 11 recurrent,19 single and 17 multiple fuci. Results Livin mRNA was expressed in 28 of 36 TCC cases （78%）.The positive rate of Livin expression in T₁,T₂^T₄,Ⅰ,Ⅱ,Ⅲ,single,multiple,primary, recurrent was 82%（18/22）,71%（10/14）,70%（7/10）,83%（19/23）,67%（2/3）,79%（15/19）,76%（13/17）,80%（20/25）,73%（8/11）.Livin showed no correlated with the grade,stage,focus number and recurrent status.There was no expression in control group. The positive rate of Livin between TCC and control was significant. Conclusions It is indicated that Livin may play a significant role in the TCC development.Livin might be a biomarker for the detection of TCC and served as a new target for apoptosis-inducing therapy of TCC.更多还原