【摘要】 目的探讨p53,VEGF,E-cadherin(E-cad),Cytokeratin20(CK20)在膀胱移行细胞癌组织中的表达和意义。方法采用组织微阵列技术和免疫组织化学方法,对45例膀胱癌组织标本上述蛋白表达进行研究,并以8例正常膀胱黏膜组织标本作为对照。结果45例癌组织标本中p53,E-cad,CK20异常表达率分别为100.0%,55.6%,55.6%,正常黏膜组织无异常表达,组间比较差异有统计学意义(P<0.001);正常黏膜VEGF表达率87.5%,癌组织为86.7%,组间比较差异无统计学意义(P>0.05)。p53异常表达强度、E-cad异常表达率与癌组织分级分期呈正相关,CK20异常表达率与分期呈正相关而与分级无相关性,VEGF与癌组织分期分级无相关性。结论p53,E-cad,CK20在膀胱癌的发生发展过程中起重要作用,肿瘤组织VEGF蛋白水平无明显增加。组织微阵列技术适用于大样本多指标的检测,是一种高效的研究方法。更多还原

【Abstract】 Objective To investigate the expression of p53,VEGF,E-cad,CK20 and its clinical meanings in bladder transitional cell carcinoma. Methods The specimen microarrays and immunohistochemistry were applied to detect selected cancer-related protein expression in 45 cases of bladder TCC and 8 cases of normal control. Results Abnormal expression of p53,E-cad and CK20 were detected 100.0%,55.6%,55.6% respectively in bladder TCC group,which were expressed normally in control group with significant differences.However,there was no VEGF expression differences between those 2 groups (87.5% v.86.7%).Abnormal expression intensity of p53 and expression rate of E-cad were closely related to the pathological features.CK20 expression was co-related positively with disease staging rather than grading.VEGF played less role in co-relating with pathological features. Conclusions p53,E-cad and CK20 play an important roles in the development of bladder TCC,and VEGF plays less.Microarrays is suggested to detect a large scale of specimen for multiple proteins,it is effective.更多还原