【摘要】 目的通过观察失神经大鼠阴茎海绵体肌电图(CC-EMG)的变化,探讨勃起功能的神经机制,为肌电网诊断神经性勃起功能障碍提供实验依据。方法将32只成年雄性Sprague-Dawley大鼠分为正常对照组、海绵体神经损伤组、腹下神经损伤组和盆神经损伤组,每组8只。大鼠神经损伤3周后,于清醒状态下行阴茎CC-EMG检测,比较各组皮下注射阿朴吗啡前、后阴茎CC-EMG的变化,并进行统计学分析。结果各组应用阿朴吗啡前及应用后5 min和10 min,CC-EMG组内各项观察指标两两比较,差异均无统计学意义 (P>0．05)。正常对照组使用阿朴吗啡后10 min,均方根振幅(RMS)均明显高于海绵体神经损伤组、腹下神经损伤组和盆神经损伤组(P<0．05),而在阿朴吗啡应用前和应用后5 min,H/L均明显小于海绵体神经损伤组(P<0．05);海绵体神经损伤组阿朴吗啡应用前及应用后5 min和10 min,H/L均明显高于腹下神经损伤组 (P<0．05),而在应用阿朴吗啡前,MF明显高于腹下神经损伤组(P<0．05),H/L明显高于盆神经损伤组(P <0．05);盆神经损伤组应用阿朴吗啡后10 min,MF明显高于腹下神经损伤组(P<0．05)。结论 CC-EMG可以检测出海绵体神经、腹下神经和盆神经损伤大鼠阴茎海绵体的不同肌电变化,这种肌电变化的差异可能反映了这些神经在阴茎勃起功能中的不同作用。更多还原

【Abstract】 Objective To investigate the neural mechanism of erectile function through electromyograms of the corpus cavernosum (CC-EMG) of conscious de-enervated rats. To develop an EMG diagnosis for erectile dysfunction after peripheral nerve injury. Methods 32 male adult SD rats were divided into four groups: a control group ( n = 8) , and groups in which injuries were induced in the cavernous nerve ( T9,n=8 ) , the hypogastric nerve ( L6, n =8) , and the pelvic nerve (n =8). After three weeks, all experimental rats underwent CC-EMG exam before and at different time points after hypodermical injection of apomorphine. Results No significant differences were found within each group comparing the RMS (root mean square) , MF (mean frequency) and H/L (high/low power ratio) of the EMGs. The controls showed greater RMS levels than the other three groups 10 min after apomorphine but had lower H/L values than the cavernous nerve injured group before testing and at 5 min. The rats with cavernous nerve injuries had greater H/L ratios than the hypogastric nerve injured before testing, at 5 min and at 10 min after hypodermical injection of apomorphine, and had greater MF than the hypogastric nerve injured and had greater H/L than the pelvic nerve injured beforehypodermical injection of apomorphine. The MF of the pelvic nerve injured rats was higher than among the hypogastric nerve injured 10 min after hypodermical injection of apomorphine. Conclusion It is possible to record the electrical activities of the corpus cavernosum in de-enervated rats. CC-EMG attributes could reflect different kinds of erectile dysfunction from various peripheral nerve injuries.更多还原