【摘要】 目的通过观察短波辐射对HBV转基因小鼠树突状细胞(DC)分泌白介素-12(IL-12)及T 淋巴细胞分泌IL-10、IFN-γ的影响,从而探讨短波辐射治疗慢性乙型肝炎的可能相关机制。方法选取 HBV转基因小鼠20只,将其随机分为短波组和空白对照组,另取10只健康小鼠作为正常对照组,其中短波组小鼠给予短波辐射。各组小鼠分别于实验进行10 d后取其脾脏分离树突状细胞,经流式细胞仪检测 DC表型,采用ELISA方法检测DC分泌IL-12以及在刺激混合淋巴细胞反应中T淋巴细胞分泌IL-10、IFN- γ的含量变化情况。结果短波组DC分泌IL-12水平较空白对照组显著增高(P<0．01),与正常对照组比较,差异亦有统计学意义(P<0．01);在混合淋巴细胞反应中,短波辐射能显著抑制T淋巴细胞分泌IL-10 (P<0．01),并同时提高IFN-γ水平(P<0．01)。结论短波辐射能显著上调HBV转基因小鼠DC功能,对 HBV所致慢性乙型肝炎的临床治疗具有潜在应用价值。更多还原

【Abstract】 Objective To observe the effect of short wave diathermy on the functioning of dendritic cells ( DCs) derived from the spleens of hepatitis B virus ( HBV) transgenic mice. Methods HBV transgenic mice were treated with short wave diathermy for 10 days. Dendritic cells were then separated from their spleens and incubated with granulocyte-macrophage colony-stimulating factor ( GM-CSF) and interleukin-4 ( IL-4 ). The phenotypes of the DCs were assayed by flow cytometry ( FCM) ,IL-12 released by the DCs and IL-10 and IFN-γ produced by the T cells in a mixed lymphocyte reaction( MLR) were measured using ELISA. Results Short wave diathermy could enhance the DCs’ phenotype expression, increase IL-12 levels ( P < 0. 01 ) , promote the secretion of IFN-γ,and inhibit the secretion of IL-10 in a MLR (P <0. 01). Conclusion Shortwave diathermy can significantly up-regulate DC function , so it might be a potential modality used for the treatment of chronic hepatitis B and hepatocellular carcinoma.更多还原