【摘要】 目的介绍病例同胞对照设计的研究设计及统计分析方法,并对实际资料进行统计分析。方法以亚甲基四氢叶酸还原酶基因C677T突变与冠心病连锁研究为实例,采用同胞-传递不平衡检验(s-TDT)和同胞组不平衡检验(SDT)等方法进行统计分析。结果采用s-TDT分析,统计量Z=0.27,P>0.05;采用SDT分析,χ2=0.31,P>0.05。提示受累子代与非受累子代T等位基因分布差异无统计学意义。结论病例同胞对照设计使用患者同胞作为对照,避免了人群分层现象,可以用来检测基因与疾病之间的关联或连锁。更多还原

【Abstract】 Objective To introduce the design and statistical methods of case-sibling control design and to analyze the published data. Methods Data from an association study between the coronary heart disease and methylenetetrahydrofolate reductase gene C677T polymorphism was analyzed by the sib transmission/disequibrium test (s-TDT) and the sibship disequilibrium(SDT) methods. Results Using s-TDT method,Z value was 0.27 with P> 0.05. The result of SDT method showed that chi-square was 0.31 with 1 df, P> 0.05. All results suggested that neither s-TDT nor SDT showed significant difference between the transmitted and untransmitted methylenetetrahydrofolate reductase gene C677T allele distributions. Conclusion Case-sibling control design might avoid population stratification by using siblings as controls thus might be used to test association and linkage between genes and disease.更多还原