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女性生殖道不同部位检测支原体的序贯试验分析

Sequential analysis of detection Mycoplasma in different positions of female genital tract

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【作者】 王蓓于红徐金水郭海健艾静

【Author】 WANG Bei*, YU Hong, XU Jin-shui, GUO Hai-jian, AI Jing. *Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China

【机构】 东南大学公共卫生学院流行病学与卫生统计学系东南大学附属中大医院妇产科东南大学公共卫生学院流行病学与卫生统计学系

【摘要】 目的探讨解脲脲原体和人型支原体在女性生殖道的主要寄生部位。方法采用载量一致的标准无菌棉拭子分别于女性阴道穹窿和宫颈口采样,用法国生物梅里埃ID试剂进行解脲脲原体和人型支原体的培养鉴定及半定量计数,按照双向质反应开放型序贯试验设计,对检测结果进行比较分析。结果阴道穹窿部位标本中两种支原体的总阳性数和≥104ccu/ml半定量阳性数均高于宫颈口标本。结论对女性进行生殖道支原体感染的检测时应采集阴道穹窿标本,以提高检出率。

【Abstract】 Objective To discuss the main parasitic position of Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) in female genital tract. Methods Using the standard aseptic cotton swab to collect secretion in vaginal fornix and orificium internum uteri, to culture Uu and Mh in Mycoplasma ID medium of France Bio-Merieux Co. According to double-direction quality reaction sequential test design, detection results of different position were analyzed. Results Total positive and ≥104ccu/ml positive of Uu in vaginal fornix were significantly higher than that in orificium internum uteri. Total positive of Mh in vaginal fornix was significantly higher than that in orificium internum uteri as well. Conclusion In order to raise the detectable rate of Mycoplasma, we suggested that the secretion in vaginal fornix position be collected.

【基金】 国家自然科学基金资助项目(30471488)
  • 【文献出处】 中华流行病学杂志 ,Chinese Journal of Epidemiology , 编辑部邮箱 ,2006年02期
  • 【分类号】R446.5
  • 【被引频次】8
  • 【下载频次】145
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