【摘要】 目的观察乐果染毒对大鼠血清单胺类递质去甲肾上腺素(NE)、肾上腺素(E)、5-羟色胺(5-HT)、多巴胺(DA)及其代谢物3,4-二羟苯乙酸(DOPAC)浓度变化的影响,探讨乐果中毒的非胆碱能机制。方法雄性SD大鼠104只随机分为对照组(生理盐水)、乐果低(38．9mg/kg)、中(83．7mg/kg)、高(180．0 mg／kg)3个剂量组,一次性腹腔注射染毒,给药后0．5、2、8、24 h断头处死并分离血清,用高效液相色谱电化学检测法检测血清中NE、E、5-HT、DA及其代谢物DOPAC的浓度。结果不同时程的染毒组(低、中、高剂量组)血清中DA及其代谢物DOPAC和5-HT的浓度比对照组分别增加8．42％-248．42%、17．22％-68．21％。不同时程的染毒组血清NE和E的浓度比对照组分别减少9．65％-38．26％和11．00％-32．62％。5-HT浓度开始随染毒剂量增加而减少,但随染毒时程的增加,则随染毒剂量增加而增加。DA、DOPAC、NE、E和5-HT的浓度在不同时程的低、中、高剂量组间差异均有统计学意义(P<0．05)。结论DA、DOPAC和5-HT浓度有随染毒剂量和时程的增加而增加的趋势,NE和E浓度有随染毒剂量和时程的增加而减少的趋势,存在剂量时程效应关系。更多还原

【Abstract】 Objective To study the effect of dimethoate on the monoamine neurotransmitters, including norepinephine(NE), epinephine(E), serotonin(5-HT), dopamine(DA) and its metabolite (3,4-hydrox-yphenylaceticacid, DOPAC) in the serum of rats and furthermore to explore the non-cholinerigic mechanism of organophosphate induced toxicity. Methods Groups of rats were treated with saline and 38.9, 83.7 and 180.0 mg/kg dimethoate respectively and were decapitated at the different time course from 0.5 to 24 hours after the administration. The monoamine neurotransmitters were determined by the reverse-phase high-performance liquid chromatography with the electrochemical detection. Results The serum concentrations of DA (8.42% -248.42% of the control), DOPAC (17.22% - 68.21% of the control) increased, according with the dimethoate dosage and the exposure time, while the levels of NE (9.65% - 38.26% of the control) and E (11.00% -32.62% of the control) contents decreased at the same time. Conclusion Dimethoate induced toxic effects can alter the monoamine levels at the different dosage and the time exposure in the serum of rats. It suggests that some non-cholinergic mechanisms may be involved in the dimethoate intoxication.更多还原