【摘要】 目的研究GSM 1800 MHz射频电磁场(以下简称“射频场”)对细胞膜表皮生长因子(EGF)受体聚簇的可能诱导作用及噪声磁场的干预。方法将中国仓鼠肺成纤维细胞(CHL)分别用1800 MHz射频场(包括217和50 Hz调制和非调制)、噪声磁场和射频场与噪声磁场叠加的复合场处理15 min,用EGF处理作为阳性对照,射频场的比吸收率(SAR)值取0．1、0．5、1．0、2．0和4．0W/kg。上述处理后的细胞经间接免疫荧光染色标记后,用激光共聚焦显微镜观察其细胞膜EGF受体的聚簇现象。结果SAR值为0．5、1．0、2．0和4．0 W/kg的调制射频场辐照CHL细胞15 min可诱导细胞膜EGF受体的聚簇,但当SAR值为0．1 W/kg时,细胞膜不出现EGF受体聚簇。而非调制射频场以及2μT噪声磁场不能诱导细胞膜EGF受体的聚簇,当2μT噪声磁场与射频场叠加后,可抑制射频场诱导的细胞膜EGF受体聚簇。结论GSM1800MHz射频场能诱导细胞膜EGF受体的聚簇,波的调制在其中起主要作用；一定强度的噪声磁场可阻断GSM 1800 MHz射频场诱导的细胞膜EGF受体聚簇。更多还原

【Abstract】 Objective To investigate the possible effect of exposure to GSM 1 800 MHz radiofrequency electromagnetic fields(RF EMF) on epidermal growth factor(EGF) receptor and its possible interference by noise magnetic fields(MF).Methods Chinese hamster lung fibreblasts(CHL) were exposed to 1 800 MHz RF EMF (modulated by 217 Hz or 50 Hz,or unmodulated),2μT noise MF,and RF EMF combined with 2μT noise MF for 15 min,respectively.The specific absorption rates(SARs) of RF EMF were 0.1,0.5,1.0,2.0 and 4.0 W/kg. Commercial EGF(1 ng/ml) treatment was used as positive control.EGF receptors on the cell membrane were ob- served under a laser scanning confocal microscope after indirect immunofluorescence staining.Results EGF re- ceptor clustering was induced after exposure to GSM 1800 MHz RF EMF modulated by 217 Hz or 50 Hz MF at SARs of 0.5,1.0,2.0,4.0 W/kg for 15 min as induced by 1 ng/ml EGF,but not at SAR of 0.1 W/kg.And no EGF receptor clustering was found in cells after exposure to unmodulated RF EMF or 2μT noise MF.In addition, snperposition of 2μT noise MF could inhibit the EGF receptor clustering induced by GSM 1 800 MHz RE EMF. Conclusion EGF receptor clustering in CHL cells can be induced by GSM 1 800 MHz RF EMF at the lowest SAR of 0.5 W/kg and inhibited by noise MF.The modulation of wave may play an important role in the inducement of receptor clustering after RE exposure.更多还原