【摘要】 目的探讨在百草枯（paraquat,PQ）中毒所致的大鼠急性肺损伤时黄芩甙（baicalin,Bai）对肺组织损伤和肺间质巨噬细胞肿瘤坏死因子（TNF-α）表达水平及肺组织中血红素氧合酶-1（HO-1）表达的影响。方法将SD大鼠随机分为对照组、PQ染毒组、Bai治疗组和Bai单纯对照组,每组10只。PQ染毒组腹腔注入PQ液（25 mg/kg）制造中毒模型；Bai治疗组对造模成功大鼠静脉注射Bai(300 ng·kg^-1·d^-1),共3 d；Bai单纯对照组是健康大鼠静脉注射Bai(300 mg·kg^-1·d^-1),共3 d；对照组给予等量生理盐水。均观察3 d后,取肺组织常规HE染色和电镜观察肺组织损伤情况,分离培养肺间质巨噬细胞（PIM）观察TNF-α的表达情况。用逆转录聚合酶链反应（RT-PCR）检测肺组织的HO-1 mRNA表达。用Western blot方法分析肺组织中HO-1蛋白表达。结果对照组和Bai单纯对照组肺组织结构基本正常,PQ染毒组肺组织损伤较对照组明显加重,Bai治疗组肺组织损伤较PQ组减轻。Bai治疗组的TNF-α表达水平[（484．2±39．5）μg/L]低于PQ染毒组[（790．2±35．0）μg/L],高于对照组[（121．6±19．2）μg/L],且差异均有统计学意义（P＜0．05）,Bai治疗组肺组织的HO-1 mRNA和蛋白表达水平分别为59．8±5．40和122．0±31．98,明显高于PQ组（45．9±5．82和77．92±10．23）,差异均有统计学意义（P＜0．05）。结论Bai减轻了大鼠PQ中毒所致的肺损伤,其机制可能与PIM的TNF-α表达水平降低及肺组织中HO-1基因和蛋白表达升高有关。更多还原

【Abstract】 Objective To investigate the effect of baicalin（Bai） on lung injury,the level of TNF-αin cul- tured liquid of pulmonary interstitial macmphage and the expression of heme oxygenase-1 （HO-1） in lung injury as- sociated with paraquat poisoning.Methods Rats were randomizedly divided into four groups:control group,PQ group,Bai group(Bai,300 mg·kg^-1·d^-1)and simple Bai group(Bai,300 mg·kg^-1·d^-1)（n=10 in each group）.The 2% PQ was injected（25 mg/kg） in PQ group.Bai was injected in the rats(300 mg·kg^-1·d^-1×3d) through caudal vein after paraquat poisoning in Bai group.In simple Bai group,Bai was injected in the healthy rats (300 mg·kg^-1·d^-1×3d).The samples were obtained three days after intraperitoneal administration of 2% paraquat（25 mg/kg）.The injury of lung was estimated with HE dyeing and electron microscope.Pulmonary intersti- tial macmphage（PIM） were obtained,and then cultured for 24 hours.The content of TNF-αwas evaluated.The ex- pression of HO-1 mRNA was detected by reverse transcription polymerase chain reaction（RT-PCR）.The expression of HO-1 protein was evaluated by Western blot analysis.Results The lung tissue was normal in control group and simple Bai group.The degree of lung injury in PQ group was higher than that in control group by HE dyeing and electron microscope observation.The level of TNF-αexpression in cultured PIM in Bai group[（484.2±39.5）μg/ L] was lower than that in PQ group[（790.2±35.0）μg/L],but higher than that in the control group[（121.6±19.2）μg/L]（P＜0.05）.The expression of HO-1 mRNA and protein[（59.8±5.40）and（122.0±31.98）]in Bai group were higher than those in PQ group[（45.9±5.82）and（77.92±10.23）]（P＜0.05）.Conclusion The hmg injury associated with paraquat poisoning was alleviated by baicalin,which was possibly related to the de- crease of level of TNF-αin cultured PIM and the increase of the expression of HO-1 mRNA and protein.更多还原