【摘要】 目的建立神经生长因子(NGF)对2,5己二酮(HD)诱导的PC12细胞凋亡保护作用的模型,并探讨其作用机制。方法以PC12细胞为神经元的细胞模型,将HD和不同浓度的NGF加入培养的PC12细胞中,四甲基偶氮唑盐检测细胞活性,琼脂糖凝胶电泳检测DNA断裂,流式细胞仪检测细胞凋亡率,Westernblot检测p53蛋白表达,比较各组间的差异。结果随NGF浓度增加,细胞存活率升高,差异有统计学意义(P<0.01);DNA断裂减少;细胞凋亡率减小(P<0.01);p53表达量下降(P<0.05)。结论NGF对PC12细胞可能具有直接的神经营养作用,且可保护PC12细胞免于2,5HD诱导的损伤以及具有抗凋亡作用。更多还原

【Abstract】 Objective To explore whether the nerve growth factor has protective effects on PC12 cells from injury induced by 2,5-hexanedione. Methods With PC12 cells as the model of neurons,different concentrations of NGF were added into the culture of PC12 cells.Then cell viability was tested with MTT.The DNA fragment was observed with agarose gel electrophoresis.The apoptosis ratio was tested with flow cytometry (FACS).The p53 protein was detected with western blot.The differences among the groups were compared. Results Cell viabilities were increased with the increase of the concentrations of NGF (P<0.05).The DNA fragment,the apoptosis ratio and the expression of p53 were all decreased with the increase of the concentrations of NGF(P<0.05). Conclusion The NGF might have direct nutritional effects on PC12 cells,and protect them from injury induced by 2,5-HD.Moreover,it might also have anti-apoptosis effect to some extent.更多还原