【摘要】 目的探讨hMTH1Val83Met、hOGG1Ser326Cys和hMYHHis335Gln基因多态性与慢性苯中毒发病风险的关系。方法采用病例对照设计,以152名苯中毒工人为病例组,152名接触苯而没有中毒表现的工人为对照组。应用聚合酶链反应-限制性片断长度多态性分析技术(PCRRFLP)检测hMTH1c.83、hOGG1c.326和hMYHc.335位点的多态性。结果携带hMTH1c.83ValMet+MetMet和hOGG1c.326CysCys基因型的个体发生慢性苯中毒的风险性分别是携带hMTH1c.83ValVal和hOGG1c.326SerCys+SerSer基因型个体的2.51倍(ORadj=2.51,95%CI:1.14～5.49,P=0.02)和2.49倍(ORadj=2.49,95%CI:1.52～4.07,P<0.01);相比于同时携带hOGG1c.326CysCys和hMYHc.335HisHis基因型的个体,同时携带hOGG1c.326SerCys+SerSer和hMYHc.335HisGln+GlnGln两种基因型的个体有较低的慢性苯中毒发病风险(ORadj=0.33,95%CI=0.15～0.72,P=0.01);在经常吸烟的人群中,携带hMYHc.335HisGln+GlnGln基因型的个体慢性苯中毒的发病风险较携带hMYHc.335HisHis基因型的个体降低(ORadj=0.15,95%CI:0.03～0.68,P=0.01)。结论hMTH1Val83Met和hOGG1Ser326Cys多态可能与个体慢性苯中毒的发病风险改变有关,而hOGG1Ser326Cys和hMYHHis335Gln基因多态之间可能存在潜在的联合作用。更多还原

【Abstract】 Objective To explore the relationship between genetic polymorphisms in hMTH1,hOGG1 and hMYH and risks of chronic benzene poisoning(CBP). Methods A case control study was conducted.One hundred and fifty-two BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated.The polymerase chain reaction restrainedfragment length polymorphism technique(PCR-RFLP) was applied to detect the single nucleotide polymorphisms(SNPs) on c.83 of hMTH1 gene,c.326 of hOGG1 gene and c.335 of hMYH gene. Results There were 2.51 times(OR_ adj=2.51,95%CI:1.14～5.49,P=0.02) and 2.49 times(OR_ adj=2.49,95%CI:1.52～4.07,P<0.01) risks of BP for individuals carrying genotypes of hMTH1c.83Val/Met+Met/Met or hOGG1c.326Cys/Cys compared with individuals carrying genotypes of hMTH1c.83Val/Val or hOGG1c.326Ser/Cys+Ser/Ser,respectively.Compared with individuals carrying genotypes of hOGG1c.326Cys/Cy and hMYHc.335is/His at the same time,there was 0.33 times(OR_ adj=0.33,95%CI=0.15～0.72,P=0.01) risks of BP for these with genotypes of hOGG1c.326Ser/Cys+Ser/Ser and hMYHc.335His/Gln+Gln/Gln simultaneously.In the smoking group,there was 0.15 times(OR_ adj=0.15,95%CI: 0.03～0.68,P=0.01) risks of BP for subjects carrying genotypes of hMYHc.335His/Gln+Gln/Gln compared with these carrying genotypes of hMYHc.335His/His. Conclusion Polymorphisms of hMTH1 Val83Met and hOGG1 Ser326Cys may contribute to altered risks of CBP,and potential interaction may exist among polymorphisms of hOGG1 Ser326Cys and hMYH His335Gln.更多还原