【摘要】 目的建立利用气相色谱-质谱(GC-MS)联用技术准确测定人尿中柯铁宁含量的方法。方法用β-葡萄糖苷酸酶水解共轭存在的三羟基柯铁宁(THOC)及柯铁宁(COT),以n-丁基乙酸及二氯甲烷混合液(2:1)萃取尿中的柯铁宁成分。应用HP-5MS交联石英毛细管柱进行分离,用GCMS 测定其含量。结果该方法检出限为0．02μg/L,回收率>90％,平均相对标准偏差为4．30％。结论 GC-MS法可以准确测定人尿中柯铁宁含量,满足痕量分析的要求,并具有背景值少、快速、准确可靠等特点,已应用于广州生物库老年人队列大样品量的人尿中柯铁宁的研究。更多还原

【Abstract】 Objective To establish the method of gas chromatograph-mass spectrometry (GC-MS) for the determination of the cotinine(COT) in human urine. Methods The conjugated trans-3’-hydroxycotinine(THOC) and COT were hydrolyzed in human urine with β-glucuronidase. The composition of COT was extracted with the mixture of dichloromethane and n-butyl acetate (2:1) and was separated with HP-5MS .fused-silica capillary column.The GC-MS was used for determining its content. Results The monitoring limit of this method was 0.02 μg/L. Its recovery rate was higher than 90%, Its accuracy rate was 4.30% . It was used for the determination of the cotinine in human urine in Guangzhou Biological Bank the Elderly Cohort. Conclusion The GC-MS method is a good microanalysis for monitoring the cotinine in human urine rapidly and accurately with little background disturbance. It has been applied in our Guangzhou Cohort Study for determining cotinine in human urine.更多还原