【摘要】 目的探讨雌激素受体(estrogenreceptors,ER)及雌激素相关受体α(estrogenrelated-re-ceptorα,ERRα)基因在骨外膜来源成骨样细胞的表达及其与雌激素调控的关系。方法6周龄、雌性Wistar大鼠颅骨骨外膜来源的成骨样细胞分别在含有0.01、0.05、0.1、0.25、0.5、0.75、1、5、10nmol/L9种浓度17β-雌二醇(17β-E2)或对照的成骨诱导培养液中培养7d,提取细胞总RNA,采用RT-PCR法分析ER及ERRα基因的表达情况。结果ERα基因表达阴性,ERβ基因呈弱表达。从0～0.25nmol/L范围内可以看到ERβ的表达水平有上升趋势。5nmol/L和10nmol/L雌激素组ERβ的表达水平显著低于其他雌激素组(P<0.01)。ERRα表达阳性,各雌激素组的表达水平均显著高于对照组(P<0.01)。0.25nmol/L组的ERRα表达水平最高、0.5nmol/L组次之,两组的表达水平显著高于其他雌激素组(P<0.05);10nmol/L组表达水平显著低于其他雌激素组(P<0.05)。结论雌激素上调了骨外膜来源成骨样细胞ERRα的表达水平,低水平的雌激素有可能上调ERβ的表达水平。血清17β-E2水平维持在早、晚卵泡期的生理浓度有可能获得最佳的骨保护疗效。更多还原

【Abstract】 Objective To study the gene expression of estrogen receptors(ER)and estrogen related-receptor α(ERRα)in the periosteum-derived osteogenic cells and its relationship with the regulation of estrogen.Methods The 6-week-old female Wistar rat calvaria periosteum-derived osteogenic cells were divided 10 groups and cultured in different medium for 7 days.One group severved as control group,and cultured in osteogenic inducing medium.The other 9 group severved as experimental group,and the culture medium contained 0.01,0.05,0.1,0.25,0.5,0.75,1,5,10 nmol/L 17β-estradiol(17β-E2).All RNA was extracted,and then using RT-PCR assay to determine the effects of 17β-E2 on the mRNA expression level of ER and ERRα.Results ERα expression was negative.ERβ expression level was relatively low.There was gradually increasing trend for the expression level of ERβ from 0 to 0.25 nmol/L 17β-E2 treatment.The ERβ expression level of 5 and 10 nmol/L groups was significantly lower than the other 17β-E2 treatment groups.ERRα expression was positive.The expression level of 17β-E2 treatment group was significantly higher than control group(P< 0.01).0.25 nmol/L group has the highest expression level,followed by 0.5 nmol/L group.Both of them have significantly higher expression level than the other 17β-E2 treatment groups.The expression level in 10 nmol/L group significantly lower than that in the other 17β-E2 treatment groups.Conclusion Estrogen may increase the ERRα expression level of periosteum-derived osteogenic cells.Low concentration of estrogen may increase the ERβ expression level.Maintaining serum 17β-E2 concentration at physiological level of the early and late follicular phase may obtain the optimal bone-protecting effect.更多还原