【摘要】 目的探讨小鼠骨髓基质细胞放射损伤后Notch信号的表达。方法应用小鼠骨髓基质细胞体外放射损伤模型,采用RT-PCR检测了小鼠骨髓基质细胞Notch1、Jagged1、Hes1基因的表达。结果正常小鼠骨髓基质细胞中未见Notch1受体、Notch1配体Jagged1、Notch通路活化的下游基因Hes1mRNA的表达,给予60Coγ射线照射后2h即可见上述基因的表达,照射后4h达高峰,8~12h恢复正常。各组间比较,差异有统计学意义(P<0·001)。结论γ射线作用于小鼠骨髓基质细胞后有Notch通路的活化。Notch通路参与了小鼠骨髓基质细胞的放射损伤过程。更多还原

【Abstract】 Objective To investigate the change of Notch expression in murine bone marrow stromal cell after exposure to γ-rays in vitro. Methods Cultured murine bone marrow stromal cells irradiated with 8.0 Gy 60 Co γ-rays were used in this study. The Notch₁, Jegged₁, Hes₁ genes expressions were analyzed by RT-PCR at 2 h, 4 h, 8 h, and 12 h after the irradiation. Results There were no expression of Notch₁, Jegged₁, and Hes₁ genes in normal murine bone marrow stromal cells at mRNA level, while Notch₁, Jegged₁, and Hes₁ genes were expressed at 2 h after the irradiation, and reached a peak at 4 h, then returned back to the normal level at 8-12 h. The differences between the exposed group and the control group were statistically significant （P<0.001）. Conclusions Notch signaling pathway was activated in cultured murine bone marrow stromal cellls after exposure to γ-rays, it is suggested that Notch signaling pathway is involved in the radiation damage to murine bone marrow stromal cells.;更多还原