【摘要】 目的探讨抗癌活性肽(anti-cancer bioactive peptide,ACBP)肝肽和科脾肽对人鼻咽癌细胞株CNE体外增殖抑制作用及细胞周期的影响。方法将人鼻咽癌CNE细胞与不同浓度的ACBP进行体外培养,采用四甲基偶氮唑蓝法测定不同浓度的ACBP对CNE细胞的生长抑制率;光镜下观察形态学变化;流式细胞仪分析细胞周期与凋亡率。结果质量浓度5．0-20．0μg／ml的ACBP均能抑制CNE细胞的生长,且随浓度及作用时间延长抑制率上升,20．0μg／ml ACBP作用72 h后,肝肽组的抑制率为63．0％,科脾肽组抑制率为46．7％。光镜下可观察到ACBP作用后的细胞凋亡现象。流式细胞仪结果显示:5．0μg/ml肝肽和科脾肽作用CNE细胞24 h的早期凋亡率较高,分别为(11．8±0．3)％和(8．1±0．2)％,分别与对照组比较,差异均有统计学意义(t值分别为42．535和47．300, P值均为0．000);20．0μg／ml的肝肽和科脾肽作用CNE细胞,随作用时间延长S期细胞构成比有明显上升。结论肝肽和科脾肽对CNE细胞均有抑制增殖的作用,其抗鼻咽癌细胞增殖作用机制可能为诱导肿瘤细胞凋亡,调控肿瘤细胞周期。更多还原

【Abstract】 Objective To investigate effects of two kinds of anti-cancer bioactive peptide ( ACBP) on proliferation and cell cycle in human nasopharyngeal carcinoma strain CNE. Methods Cell culture was used in vitro, CNE cells were exposed to different concentration ACBP, in all groups, contrast groups were set up. And 24,48,72 hours later, growth characteristics of CNE cells were studied by morphological observation and MTT assay . Cell cycle and apoptosis were analyzed by flow cytometry (FCM). Results In normal contrast group, CNE cells grew intensively and contacted with each other. However, cells which were treated with ACBP were inhibitory greatly in higher dose ACBP group, necrosis could be found . MTT assay showed that ACBP inhibited growth of CNE cell. FCM showed that ACBP (20. 0μg/ml ) could raise cell ratio of S phase and induce apoptosis of CNE cells. CNE cells were treated by two kind of ACBP (5.0μg/ml) for 24 h, FCM showed that early apoptosis rate were(11.8±0.3)% and(8. 1±?.2)% respectively, which showed statistical significance in comparison with control group (t = 42. 535,47. 300 respectively, P = 0. 000) . Under light microscope, some sings of cell apoptosis including coagulation of chromatin, gragmentation of nuclei and apoptotic body could be found. Conclusions Two kinds of ACBP inhibited human nasopharyngeal carcinoma strain CNE proliferation and arrested the cells to S phase, also induced the cells to apoptosis.更多还原