【摘要】 目的探讨多孔丝素材料用于面神经缺损修复的可能。方法以多孔丝素导管修复SD大鼠面神经5 mm缺损,设壳聚糖导管为对照。术后4、6、8周行电生理学检查,2、4、6、8周取材HE及甲苯胺蓝染色组织学检查,以了解神经再生及功能恢复情况。结果随时间推移,大鼠神经得以成功再生,8周时多孔丝素导管组的再生神经动作电位平均振幅已达健侧的24．9％±5．7％(x±s,以下同),与壳聚糖导管组(13．7％±3．4％)比较差异无统计学意义(P=1．125);8周时丝素导管组有髓神经纤维平均计数(62．5±6．3)与壳聚糖导管组(55．3±8．4)比较差异有统计学意义(P= 0．016)。结论多孔丝素导管对面神经缺损能起到一定作用,有可能促进和引导神经再生。更多还原

【Abstract】 Objective To evaluate the feasibility of using polyporous silk fibroin as a kind of novel material for facial nerve regeneration. Methods The porous silk fibroin conduit was used in the reconstruction of a 5 mm facial nerve gap of SD rat. Chitosan conduit was taken as control group. General observation, electrophysilogical study, histological study and image analysis were performed 2,4,6 and 8 weeks postoperatively. Results The facial nerve of SD rat regenerated successfully as time passed through. Mean CAP percentage of regenerated nerve in SF conduit was 24.94%±5.73% 8 weeks postoperatively, which had no statistical significance with that of chitosan conduit group(P = 1. 125). And the average number of myelinated myelinated nerve fibers in SF conduit was 62. 5±6. 3, which had statistical significance with that in chitosan conduit group ( P = 0. 016 ) . Conclusions The porous silk fibroin conduit could effectively repair facial nerve defect and improve peripheral nerve functional recovery.更多还原