【摘要】 目的探讨实验性脊髓损伤中低氧诱导因子1α(HIF-1α)调控的两种糖酵解酶——醛缩酶A(ALDA)和M型乳酸脱氢酸(LDH-M)在损伤脊髓中的活性变化趋势及其意义。方法SD大鼠随机分为损伤后12h、1,2,3d、1周和2周以及正常对照组,各损伤组以脊髓压迫模型致伤。采用酶组织化学方法,观察伤后各时段损伤脊髓中ALDA和LDH-M的酶活性变化情况。结果ALDA从伤后2d到伤后1周吸光度(A)值显著下降(P＜0.05),到伤后2周才逐渐下降；LDH-M则从伤后1d开始出现A值的显著下降(P＜0.05),至伤后2周恢复正常。结论损伤脊髓中受HIF-1α调控的ALDA和LDH-M酶活性显著增高。更多还原

【Abstract】 Objective To investigate the activity change pattern and corresponding significance of gly- colytic enzymes including alsolase A(ALDA)and lactate dehydrogenase M(LDH-M)regulated by hy- poxia-inducible factor 1α(HIF-1α)in spinal cord injury(SCI)of rats.Methods SD rats were ran- domly divided into control group and groups at 12 hours,1,2 and 3 days,1 and 2 weeks after compres- sive SCI,in which the activity changes of ALDA and LDH-M in the injured spinal cord were observed at different time points by means of enzyme histochemistry.Results Opitical density(A)value of AL- DA continued significant increase from two days to one week after SCI(P＜0.05)and decreased gradual- ly at 2 weeks after SCI.A value of LDH-M began significant increase at day 1 after SCI and recovered to normal level at 2 weeks after SCI(P＜0.05).Conclusion Activities of ALDA and LDH-M regulated by HIF-1αin spinal cord injury is significantly increased.更多还原