【摘要】 目的了解广州某部新兵连戊型病毒性肝炎(戊型肝炎)暴发流行的分子病毒学特征,并与当地散发毒株比较,以查找病原可能来源。方法用逆转录聚合酶链反应(RT-PCR)法,对抗HEV-IgM阳性的34例暴发性戊型肝炎及46例散发性戊型肝炎患者的血清和粪便标本进行HEVRNA检测,并对HEVRNA阳性标本的基因开放读码框(ORF)2部分片段进行克隆测序分析。结果34例暴发流行病例标本中检测出12株病毒,46例散发病例标本中检到2株。经克隆测序分析,各暴发毒株的核苷酸同源性为95.3%~100%;氨基酸同源性为94.0%~100%。且暴发毒株和散发毒株的核苷酸及氨基酸的同源性也较高,分别为95.3%~99.3%和94.0%~100%;暴发毒株和散发毒株与各型中的标准株相比,与Jap1株同源性最高,其核苷酸同源性为92.0%~95.3%,氨基酸同源性为96.0%~100.0%。进化树分析提示本次戊型肝炎暴发流行病毒株与戊型肝炎病毒基因Ⅳ型距离最近。结论本次戊型肝炎暴发流行的病原可能来于广州本地;广州地区戊型肝炎流行毒株属戊型肝炎基因型Ⅳ型。更多还原

【Abstract】 Objective To investigate the genotype and gene mutation of hepatitis E virus isolated from the serum and stool samples of the patients with epidemic outbreak hepatitis E in certain recruit barracks of Guangzhou. Methods The reverse transcription polymerase chain reaction (RT-PCR) was performed to amplify the partial ORF2 nucleotide acid sequences of hepatitis E virus isolated from 34 and 46 inpatients with epidemic outbreak and sporadic hepatitis E respectively. The PCR products of the positive samples were cloned and sequenced. Results The 14 strains, including 12 epidemic strains and 2 sporadic strains, were isolated from the total 80 inpatients. The homology of nucleotide acid and amino acid sequences of 12 epidemic outbreak strains is 95.3%～100% and 94.0% ～100% . The homology between epidemic outbreak strains and sporadic strains is 95.3%～99.3% and 94.0%～100%. Compared with the standard different genotypes of HEV, these strains have the highest homology to the Jap1 strain which belongs to genotype Ⅳ, with homology of 96.0%～100%, respectively. Phylogenetic analysis indicated that these the nucleotide acid sequence homology of 92.0%～95.3% and amino acid sequence stains and Jap1 strain share the same cluster. Conclusion[KG1]Epidemic outbreak strains isolated from the patients in recruit barracks of Guangzhou belong to the genotype Ⅳ of HEV and the nucleotide acid sequences had partial mutation.更多还原