【摘要】 目的设计一种新型生物型人工肝,评价其治疗暴发性肝功能衰竭的疗效。方法采用无纺布编织型生物反应器接种原代猪肝细胞构建生物型人工肝,对D-氨基半乳糖胺诱导的猪暴发性肝功能衰竭模型进行治疗。结果生物型人工肝治疗可显著降低受试动物血氨和血清L-乳酸浓度,稳定血糖水平,改善Fischer指数,并延长动物存活时间。结论以无纺布编制型生物反应器为基础构建的生物型人工肝对暴发性肝功能衰竭具有明显的肝支持作用,具有临床应用可行性。更多还原

【Abstract】 Objective In this paper, the design of a bioartificial liver (BAL) based on non-woven fiber bioreactor is described and evaluated using a pig fulminant liver failure model. Methods Fulminant liver failure (FHF) was induced with D-galactosamine hydrochloride in a dose of 1.3 g/kg body weight in each pig. 12 hours later, the pigs were divided into three groups: (1) a BAL group (n=5), in which pigs received BAL treatment with 1.0 to 1.3—109 hepatocytes for a duration of 6 hours, (2) a sham BAL group (n=5), in which pigs received BAL treatment without hepatocytes, and (3) a FHF group (n=5), in which pigs only received intensive care. Parameters related to liver function and animal survivals up to 168 h were determined. Results In the BAL group, blood ammonia and serum L-lactate levels were lower, and serum glucose levels and Fischer index were higher than those in the other two groups. The survival time of FHF pigs in the BAL group was significantly prolonged (106±23 h) compared with the sham BAL (43±8 h) and the FHF group (48±7 h). Conclusions The non-woven fabric bioreactor based BAL system appears to be effective in treatment of FHF in pigs.更多还原