【摘要】 目的探讨结核分枝杆菌感染与结节病发病的关系。方法用即时荧光聚合酶链反应法检测了22例结节病患者活检样本中特异性结核分枝杆菌复合物IS6110DNA片段。结果22例结节病患者中,检测阳性11例。聚合酶链反应扩增所得到的IS6110DNA片段,经进一步测序证实与结核分枝杆菌基因序列完全一致。结论结核分枝杆菌感染可能是结节病的重要病因之一。更多还原

【Abstract】 Objective To study the role of Mycobacterium tuberculosis in the pathogenesis of sarcoidosis. Methods Archival material of 22 patients with a histologic diagnosis of sarcoidosis were retrieved. Real-time fluorescent polymerase chain reaction (PCR) was used to detect DNA fragments of the complex-specific insertion sequence IS6110 of Mycobacterium tuberculosis in formalin-fixed and paraffin-embedded biopsy samples. Results Among the 22 samples studied, Mycobacterium tuberculosis DNA was detected in 11 cases. The sequence of PCR amplified IS6110 DNA fragments completely matched with the related sequence in Mycobacterium tuberculosis gene. Conclusions Mycobacterium tuberculosis DNA is identified in a certain proportion of patients with a clinicopathologic diagnosis of sarcoidosis. Mycobacterium tuberculosis may be an important etiologic agent, at least in some of these patients.更多还原