【摘要】 目的观察TEIF蛋白在软组织肉瘤组织中的表达及与类型、分级的关系。方法通过原核表达TEIF蛋白免疫制备多克隆抗体,免疫印迹鉴定。以组织芯片-免疫组织化学(EnVision法)检测166例软组织肉瘤和28例良性肿瘤及瘤样病变标本TEIF蛋白表达。结果制备多克隆抗体经Western印迹鉴定与TEIF具有特异结合性。免疫组织化学显示166例软组织肉瘤中TEIF蛋白阳性97例,阳性率为58%(97/166),高于良性肿瘤及瘤样病变(11%,3/28)(P<0.05)。阳性主要分布为滑膜肉瘤94%(16/17)、原始神经外胚叶瘤(PNET)91%(21/23),两者高于隆突性皮肤纤维肉瘤43%(6/14)、黏液性纤维肉瘤38%(6/16)、恶性外周神经鞘膜瘤36%(8/22)、脂肪肉瘤32%(6/19)(P<0.05),而与恶性纤维组织细胞瘤75%(15/20)、横纹肌肉瘤(7/10)、平滑肌肉瘤64%(9/14)差异无统计学意义(P>0.05)。同时,TEIF蛋白的弥漫阳性表达(≥2+)多存在于PNET和滑膜肉瘤,分别为83%(19/23)和76%(13/17)。在法国癌症中心联盟(FNCLCC)分级中,19例Ⅰ级肉瘤中TEIF阳性表达为32%(6/19),44例Ⅱ级肉瘤阳性率为48%(21/44),两者中2+以上阳性分别为11%(2/19)、32%(14/44),而70例Ⅲ级肉瘤中TEIF阳性表达为84%(59/70)和2+以上阳性为70%(49/70)。Ⅲ级组的TEIF阳性率(及2+以上阳性率)显著高于Ⅱ级组和Ⅰ级组(均为P<0.05),而后两者间则差异无统计学意义(P>0.05)。结论TEIF蛋白表达存在于软组织肉瘤,尤其高表达于PNET、滑膜肉瘤,并与肉瘤的组织学分级有关。更多还原

【Abstract】 Objective To evaluate the expression of TEIF protein in human tumors of soft tissue and its significance. Methods Anti-TEIF polyclonal antibody was generated by immunization of E.coli expressed His-TEIF protein. The expression of TEIF in 166 cases of sarcomas and 28 case benign tumors or tumor-like lesions of soft tissue arranged in tissue chip was analyzed by immunohistochemistry. Results Polyclonal antibody obtained from immunized rabbit was verified in Western blot to prove its specific reactivity with native TEIF protein. The immunohistochemical staining of TEIF showed that about 58% (97/166) of sarcomas were positive and significantly different from that of benign tumors or tumor-like lesions (11%, 3/28). The positive staining was predominantly in synovial sarcoma 94%(16/17), primitive neuroectodermal tumor (PNET) 91% (21/23), both of which were significantly higher than 43%(6/14) of dermatofibrosarcoma protuberans, 38%(6/16) of myxofibrosarcoma, 36% (8/22) of malignant peripheral nerve sheath tumor, 32%(6/19) of liposarcoma, (P<0.05, respectively), but not higher than 75% (15/20) of malignant fibrous histiocytoma, 70%(7/10) of rhabdomyosarcoma or 64%(9/14) of leiomyosarcoma. Meanwhile, strong positive staining of TEIF (≥2+) was frequently observed in PNET(83%, 19/23) and synovial sarcoma (76%, 13/17). With respect to FNCLCC grading, 19 cases of grade Ⅰ sarcoma TEIF was 32%(6/19) and strong positive was 11%(2/19),44 cases of grade Ⅱ sarcoma was 48%(21/44) and 32%(14/44), and 70 of grade Ⅲ was 84%(59/70) and 70%(49/70). The rate of either positive or strong positive in grade Ⅲ sarcoma was significantly different from that of either grade Ⅰ or Ⅱ (P<0.05), but no difference between the latter two groups (P>0.05). Conclusions TEIF protein could be detected in large part of soft tissue sarcomas, and it not only over-expressed in most of PNET, synovial sarcomas, but also correlated with histological grading.更多还原