【摘要】 目的探讨 Xklp2靶蛋白(targeting protein for Xklp2,TPX2)在肺鳞癌及其癌前病变中的表达和意义。方法 Western blot 分析 TPX2蛋白在2株肺鳞癌细胞系和4株永生化支气管上皮细胞系中的表达。逆转录聚合酶链反应分析21对新鲜肺鳞癌组织及其远端正常肺组织 TPX2的 mRNA表达水平。构建肺癌组织微阵列,针对其中的319例肺鳞癌患者的组织样品及其相应的114例癌前病变组织的常规石蜡切片进行 TPX2免疫组织化学(SP 法)染色,结果与临床病理参数比较分析。结果 TPX2蛋白在被测肺鳞癌和永生化支气管上皮细胞系中均有水平不一的表达。在新鲜组织标本中76.2%的肿瘤组织较正常组织 TPX2 mRNA 明显高表达。免疫组织化学分析结果显示,TPX2蛋白的表达在肿瘤组织(64.2%)中明显高于正常组织,且与肿瘤组织病理学分级、临床分期及淋巴结转移相关。TPX2蛋白在肺鳞癌的癌前病变中的表达显著高于正常组织,且随支气管上皮病变程度加重(鳞状化生、不典型增生、原位癌)而增高。结论 TPX2蛋白的表达有可能促进支气管上皮癌变和肺鳞癌进展,而且是肺鳞癌淋巴转移的危险因素。TPX2有望成为监测肺鳞癌发生发展的候选标志物。更多还原

【Abstract】 Objective To study the expression of targeting protein for Xklp2(TPX2)and its significance in squamous cell carcinoma(SCC)of the lung.Method Two SCC cell lines and 4 immortalized bronchial epithelial cell lines(as a precancerous model)were examined by Western blot for TPX2 expression.Reverse transcription-polymerase chain reaction analysis for TPX2 was also performed using tumor tissues from 21 patients with SCC of the lung.The expression of TPX2 was studied by immunohistochemistry(using tissue microarray)on paraffin-embedded sections of pulmonary SCC and corresponding precancerous lesions from a group of 319 patients.Results TPX2 was variably expressed in all the cell lines studied.Compared with matched controls using normal lung tissue,high level of TPX2 mRNA was detected in 16 of the 21 SCC tumor tissue samples analyzed.Immunohistochemical study showed that TPX2 was mainly present in tumor tissues but not in normal controls.The expression of TPX2 correlated with tumor grade,stage and nodal status.As for precancerous lesions,the level of TPX2 was also increased, in accordance with the degree of dysplasia.Conclusions Expression of TPX2 may play a role in carcinogenesis of bronchial epithelium and tumor progression of pulmonary SCC.It may also represent a potential biomarker for surveillance of SCC of lung.更多还原