【摘要】 目的:建立百蕊草中紫云英苷的含量测定方法。方法:采用RP-HPLC测定,C18柱,流动相为乙腈-水(23∶77),用冰醋酸调pH至3.85,流速1.0 mL.m in-1,检测波长346 nm;供试品溶液的制备采用50%乙醇提取2次。结果:测定了同一产地不同批次的百蕊草药材,紫云英苷质量分数在0.120%~0.155%。结论:建立的测定方法简便、可靠。更多还原

【Abstract】 Objective: To establish a determination method of astragulin in Thesium chinese.Method: RP-HPLC analytical method was established using a Polaris C₁₈ column and acetonitrile-water（23∶77） as the mobile phase,with a flow rate of 10 mL·min¹,detected at 346 nm.The method of sample is refluxing exation by 50% alcohol for 2 times.Result: The content of astragulin was from 0.120% to 0.155%,in different groups of T.chinese collected from the same location.Conclusion: The method was validated to show convenient and reliable.更多还原