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红花药材黄色素A含量的RP-HPLC测定及其资源的质量评价

Determination of safflor yellow A by RP-HPLC and resources quality comparison in Carthamus tinctorius

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【作者】 郭美丽张戈章伟张汉明苏中武

【Author】 GUO Mei-li,ZHANG Ge,ZHANG Wei,ZHANG Han-ming,SU Zhong-wu (College of Pharmacy,Second Military Medical University,Shanghai 200433,China)

【机构】 第二军医大学药学院第二军医大学药学院 上海200433上海200433

【摘要】 目的:为红花药材质量评价体系的建立、红花优良品种的筛选提供依据。方法:选择有代表性的红花品种22个,在不同生态地区和不同年份进行栽培试验,并采用RP-HPLC测定各品种中主要活性成分黄色素A含量,建立药材质量评价标准,比较品种间差异,考察其遗传稳定性。结果:红花不同品种黄色素A的含量在0.70%~1.85%,品种间存在非常显著差异(P<0.01),其中,鱼台红花、合肥红花、芮城红花含量居前3位。结论:红花药材中的主要活性成分黄色素A为质量评价的重要指标,鱼台红花、合肥红花、芮城红花为红花优良种质资源。

【Abstract】 Objective: To provide the basis for establishing evaluation criterion,selecting good strains and carring out good agricultural practice of the crude drug.Method: Representative 22 varieties of Carthamus tinctorius were selected and cultivated in different ecological localities and different years.And the content of safflor yellow A in their corollas were measured by RP-HPLC to compare the differences and their genetic stabilities among varieties.Result: The range of of safflor yellow A content was 0.70%-1.85% which were varied among varieties(P<0.01). The content of safflor yellow A in varieties Yutai Honghua,Hefei Honghua,Rucheng Honghua were higher than in others.Conclusion: The effective compound safflor yellow A in C.tinctorius was one of the main quality evaluation criterions.Varieties Yutai Honghua,Hefei Honghua and Rucheng Honghua were good resources.

【基金】 国家自然科学基金资助项目(30271588)
  • 【文献出处】 中国中药杂志 ,China Journal of Chinese Materia Medica , 编辑部邮箱 ,2006年15期
  • 【分类号】R284.1
  • 【被引频次】16
  • 【下载频次】347
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