【摘要】 目的:探讨大黄素对肝脏缺血-再灌注损伤的保护作用及其机制。方法:将45只成年雄性SD大鼠随机分成三组:假手术对照组(A组)15只,肝缺血30min、再灌注90min组(B组)15只,术前5d给予大黄素灌胃60mg/(kg·d),5次+肝缺血30min、再灌注90min(C组)15只;观察血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、透明质酸酶(HA)、肝组织丙二醛(MDA)含量的变化及肝脏组织病理学改变情况,同时测定各组大鼠胆汁流量情况。结果:C组与B组相比,MDA含量显著降低(P<0.05),HA浓度明显降低(P<0.01),胆汁流量显著增加(P<0.01),同时C组的肝细胞功能明显改善,且肝脏超微结构改变也较轻。结论:大黄素对肝脏缺血-再灌注损伤具有保护作用,该作用与减轻肝脏缺血再灌注后脂质过氧化程度和肝窦内皮细胞损伤有关。更多还原

【Abstract】 Objective To investigate the protective role and mechanism of emodin in hepatic ischemia-reperfusion injury in rats Methods Forty-five male SD rats were randomly divided into 3 groups (15 animals in each group). Group A rats served as sham-operated controls, group B rats underwent 90 minutes reperfusion after 30 minutes ischemia of the liver. Emodin 60mg/(kg.d) was administered to animals by oral route in group C for 5 days before ischemia. In addition to serum alanine aminotransferase (ALT),aspartate aminotransferase (AST) ,hyaluronidase (HA) were respectively measured in each group. The bile flow and levels of malondialdehyde (MDA) in hepatic tissue were also determined, the hepatic tissue was examined under light and electronic microscopies. Results After reperfusion, serum levels of ALT,AST,HA and content of MDA in liver tissue in group C were significantly decreased than those in the group B (P<0.01 or P<0.05). The bile flow in group C was significantly higher than that of the group B (P<0.01). The histologic changes of the liver tissue under light and electronic microscopies in group B was more obvious than in the group C. Conclusion Emodin can protect the liver from ischemia-reperfusion injury. The mechanism of emodin improving hepatic function may be accomplished through attenuating the degree of lipid peroxidation,and attenuating liver sinusoidal endothelial cells injury.更多还原