【摘要】 目的观察依普拉芬能否抑制17α-炔雌醇刺激子宫副作用,及对炔雌醇抗骨质疏松作用的影响。方法52只4月龄SD雌性大鼠随机分为6组:①假手术组;②去卵巢组;③高炔雌醇组(30μg·kg-1·d-1);④低炔雌醇组(6μg·kg-1·d-1);⑤依普拉芬(200mg·kg-1·d-1);⑥联合用药组(炔雌醇6μg·kg-1·d-1+依普拉芬200mg·kg-1·d-1)。实验12周,通过测量子宫内膜厚度及子宫湿重分析用药对子宫的刺激作用,测量骨小梁面积百分率(%Tb.Ar)、荧光周长百分数(%L.Pm)及破骨细胞数量(Oc.N分别反映骨量、骨形成及骨吸收的变化)。结果高、低炔雌醇组与去卵巢组比,%Tb.Ar增加,%L.Pm、Oc.N减少,子宫湿重及内膜厚度都显著增加。依普拉芬组与去卵巢组比,子宫湿重及内膜厚度、%Tb.Ar、%L.Pm无显著性差异,Oc.N减少。联合用药组与去卵巢组比,%Tb.Ar增加;与低炔雌醇组比,子宫重量、内膜厚度显著降低,联合用药组与正常对照组的子宫内膜厚度无显著性差异。结论依普拉芬可有效抑制炔雌醇对大鼠子宫刺激作用,与炔雌醇合用可有效防治骨质疏松。更多还原

【Abstract】 Objective To observe the effect of combined medication of ipriflavone(IP) and ethinylestradiol(EE) on uterus and bones in ovariectomized (OVX) rats.Methods Fifty-two female SD rats were divided into 6 groups:①Sham-OVX group;②OVX group;③High EE group (30 μg·kg~ -1 ·d~ -1 );④Low EE group (6 μg·kg~ -1 ·d~ -1 );⑤ IP group(200 mg·kg~ -1 ·d~ -1 );⑥EE+IP group (combined medication of 6 μg·kg~ -1 ·d~ -1 EE and 200 mg·kg~ -1 ·d~ -1 IP).After 12 weeks of experiment,the bone mass was measured with quantitative bone histomorphometry and the wet weight of the uterus and the endometrium epithelial thickness (EET) was obtained.Results The%Tb.Ar,uterine wet weight and EET of high and low EE groups was increased significantly in comparison with the OVX group,while no significant difference was found between the IP and OVX group.The IP+EE group showed increase in%Tb.Ar in comparison with the OVX group and decrease in uterine weight and EET in comparison with the low EE group without significant change when compared with the sham-OVX group.Conclusions IP could inhibit the stimulative effect of EE on uterus and prevent osteoporosis when given with EE.更多还原