【摘要】 目的探讨雌激素对脂多糖诱导的体外培养大鼠成骨细胞凋亡基因Bcl-2、Bax、Fas表达的影响,为明确雌激素抑制脂多糖诱导的大鼠成骨细胞凋亡的机理提供依据。方法新生SD大鼠颅骨第2·3代成骨细胞随机分为对照组、脂多糖组、脂多糖+雌激素组等3组,每组细胞分别培养24、48、72h,5、7、14d后作免疫组化染色,计数各组阳性细胞率,并比较组间的差异性。结果对照组成骨细胞有少量Bcl-2、Bax、Fas表达,脂多糖组细胞Bax和Fas表达显著升高(P<0·05),高峰期为72h,Bcl-2的表达无明显变化(P>0·05);脂多糖+雌激素组细胞Bax和Fas表达显著降低(P<0·05),高峰期仍为72h,且Bcl-2的表达显著升高(P<0·05)。结论脂多糖使成骨细胞Bax和Fas表达增强,而对Bcl-2的表达无明显影响;雌激素能抑制脂多糖增强成骨细胞Bax和Fas表达的作用,能使Bcl-2的表达明显增强,使Bcl-2/Bax比值增大从而抑制脂多糖诱导的大鼠成骨细胞凋亡。更多还原

【Abstract】 ?Objective To find the mechanism of inhibitive effect of estrogen on the expression of osteoblast apoptosis related genes Bax,Bcl-2 and Fas induced by lipopolysaccharide.Methods Osteoblasts of second or third generation from new born SD rats calvaria were randomly divided into three groups,namely control group,lipopolysaccharide,lipopolysaccharide with estrogen group.Cells of each group were incubated for 24h,48h,72h,5d,7d,14d,and then stained immunohistochemically.The rates of positive cells of each group were examined,and compared.?Results Scarce positive expression of Bax,Bcl-2 and Fas was detected in the control group.The expression of Bax and Fas were significantly increased (P<0.05) in lipopolysaccharide group with the peak value at 72h.The expression of Bcl-2 were not affected.The expression of Bax and Fas were significantly decreased(P>0.05) in lipopolysaccharide and estrogen group with the peak value at 72h.Conclusion Lipopolysaccharide can increase the expression of Bax and Fas in osteoblast which can be inhibited by estrogen.While the estrogen increases the expression of Bcl-2-leading to the suppression of osteoblast apoptosis.更多还原