【摘要】 目的:研究子宫内膜癌组织中磷酸化PKB、磷酸化Bad及Bim蛋白及mRNA表达水平,并探讨其与子宫内膜癌发生的关系以及三者间表达的相关性。方法:采用免疫组化方法检测11例正常增殖期子宫内膜、13例非典型增生子宫内膜及55例子宫内膜癌组织中p-PKB、p-Bad及Bim蛋白表达情况,以及采用逆转录聚合酶链反应检测上述子宫内膜组织中PKB、Bad及BimmRNA表达水平。结果:与正常子宫内膜组织比较,子宫内膜癌及非典型增生组织中p-PKB及p-Bad蛋白表达阳性率明显增加(P=0.001;P<0.001);而Bim蛋白的表达则显著减少(P<0.001),差异有显著性;PKBmRNA及BadmRNA在正常子宫内膜、非典型增生内膜及子宫内膜癌之间表达的阳性率无明显差异(P>0.05),只有BimmRNA在正常子宫内膜组织中表达的阳性率明显高于非典型增生内膜及子宫内膜癌组织(P=0.006)。结论:PKB途径的激活与子宫内膜癌的发生密切相关。其激活后可通过多种机制作用于仅含BH3域蛋白,抑制其促凋亡活性,导致子宫内膜细胞过度增殖以及子宫内膜癌的发生。更多还原

【Abstract】 Objective: To study the expression of p-PKB, p-Bad and Bim protein and mRNA in the tissues of endometrial carcinoma, and to probe the relationship between this expression and the occurrence of endometrial carcinoma. Methods: Expression of p-PKB, p-Bad and Bim protein were evaluated by immunohistochemistry (SP) in 55 cases of endometrial carcinoma, 13 cases of atypical endometrial hyperplasia and 11 cases of normal proliferative-phase endometrium. The content of PKB, Bad and Bim mRNA in those tissues was evaluated by RT-PCR. Results: p-PKB and p-BAD proteins were expressed more often in the tissues of endometrial carcinoma and atypical endometrial hyperplasia than in normal proliferative-phase endometrium (P=0.001; P<0.001). On the contrary, Bim protein was expressed more often in normal tissue (P<0.001). The amount of PKB mRNA and Bad mRNA in the three groups show no significant difference, but the amount of Bim mRNA in normal proliferative-phase endometrium is higher than that in endometrial carcinoma and atypical endometrial hyperplasia (P=0.006). Conclusions: The activation of the PKB pathway is closely related to the occurrence of endometrial carcinoma, and the activated PKB pathway can affect BH3-only proteins, inhibiting their pro-apoptotic ability, resulting in the occurrence of endometrial carcinoma.更多还原