【摘要】 目的:研究p-Bad112/136、p-Erk、p-Akt在乳腺癌癌变过程中,即乳腺正常组织、普通导管增生、轻中度不典型导管增生、重度不典型增生及导管原位癌、乳腺浸润性导管癌中的表达及意义。方法:用免疫组织化学S-P法检测131例乳腺石蜡包埋组织、WesternBlotting法检测27例乳腺浸润性导管癌及癌旁乳腺组织中p-Bad112/136、p-Akt、p-Erk的表达情况。用χ2和t检验等方法分析四种蛋白在乳腺癌组织中表达的意义及其与临床病理特征间的关系。结果:免疫组化结果显示在乳腺癌癌变过程中这四种蛋白表达阳性率呈递增趋势,其中四种蛋白在乳腺普通导管增生和重度不典型增生及原位癌间阳性表达率差异有统计学意义(P值分别为0.034、0.023、0.001、0.001);p-Bad112/136蛋白在重度不典型增生及原位癌组与浸润性导管癌组间差异有明显统计学意义(P=0.002,0.004)。统计分析显示在乳腺浸润性导管癌中这四种蛋白与患者的年龄、临床分期、肿瘤大小无明显关系,但均与组织学分级相关(P值分别为0.039、0.026、0.038、0.026)。同时p-Erk、p-Akt蛋白的表达与腋窝淋巴结转移有关(P值分别为0.041、0.016)。WesternBlotting结果显示四种蛋白含量在乳腺癌与癌旁乳腺组织的差异有明显的统计学意义(P值分别为0.002,0.001,0.002,0.003)。在乳腺癌组织中p-Bad112与p-Erk及p-Bad136与p-Akt蛋白表达量呈正相关性(P值分别为0.034、0.020)。结论:这四种蛋白表达水平在一定程度上提示了乳腺导管非典型增生向乳腺癌的转化潜能,并且提示MAPK/Erk及PI3-K/Akt传导路径在乳腺癌癌变过程中具有重要作用。更多还原

【Abstract】 Objective:To study the expression and significance of p-Bad,p-Erk and p-Akt in the progression of breast cancer,including normal breast tissue,ductal hyperplasia,slight and moderate atypical hyperplasia,severe atypical hyperplasia and intraductal carcinoma in situ,as well as invasive ductal carcinoma.Methods:The expression of p-Bad112/136,p-ERK,and p-AKT was examined in 131 paraffin-embedded specimens using immunohistochemical staining and in 27 frozen tissues by Western Blotting.The relationship between the expression of the four proteins and clinicopathological factors was analyzed by Chi-square test and t-test.Results:The positive rates for the expression of the four proteins showed a gradual increase in the progression of breast cancer,and there was a significant difference in the positive rates for expression of the four proteins between the severe atypical hyperplasia,intraductal carcinoma in situ and invasive ductal carcinoma(P=0.034,0.023,0.001,0.001).There was also a significant difference in the positive rate of the p-Bad112/136 expression between the common intraductal hyperplasia and invasive ductal carcinoma of the breast(P=0.002,0.004).No obviouscorrelation was found between expression of the four proteins and age,clinical stage or tumor size.However,the four proteins had correlations with histological grading(P=0.039,0.026,0.038,0.026 respectively).As histological grade increased,the expression of all four proteins decreased.p-Erk and p-Akt protein expression levels were also related to lymph node metastasis of the armpits(P=0.041,0.016).Western Blotting analysis for content of the four proteins in invaded adjacent tissue showed there was a significant increase in expression of the four proteins in breast cancer tissues compared with the adjacent breast tissues(P=0.002,0.001,0.002,0.003).There was a positive correlation in the protein content between p-Bad112 and p-Erk(P=0.034),and between p-Bad136 and p-Akt(P=0.020).Conclusion:The results strongly suggest that the levels of the four proteins indicate the potential of metastases to some extent and showed that MAPK/Erk and PI3K/Akt signaling cascades play an important role in the progression of breast cancer.更多还原