【摘要】 目的:本研究应用NS-398对肝癌细胞株进行药物干预,探讨NS-398对肝细胞癌凋亡的影响。方法:NS-398取0、25、50、75、100μmol/L5个浓度组,每个浓度组选取24h、48h、72h三个作用时间点。MTT法测定生长抑制情况,FCM检测细胞周期、凋亡率和Survivin、cyclinD1、PTEN的表达。结果:NS-398可以显著抑制BEL-7402细胞增殖,使G0/G1期比例显著降低;G2/M期比例显著增高并且诱导细胞凋亡(P<0.001),并呈时间和剂量依赖性。与对照组相比,NS-398使Survivin、cyclinD1、PTEN蛋白表达显著下调并呈时间和剂量依赖性关系(P<0.001)。结论:NS-398可诱导BEL-7402细胞凋亡,可能部分通过下调Survivin和PTEN途径实现的。更多还原

【Abstract】 Objective: To apply NS-398 for medicinal intervention of the cell lines of liver cancer and to investigate the influence of NS-398 on apoptosis of hepatocellular carcinoma. Methods: The media containing various concentrations of NS-398 (0, 25, 50, 75 and 100?滋mol/L) were used for concentration-dependent experiments. Three time-points (24h, 48h, 72h) of action time were taken for each concentration group, and the cells were collected daily for 3 days. The inhibition effect of cell growth was measured using MTT assay. Changes of cell cycle, the apoptotic cells and expression of survivin, cyclinD1 and PTEN after treatment with NS-398 were detected by flow cytometry method. Results: Treatment of with NS-398 significantly suppressed cell proliferation in time- and dose-dependent manner both. The percentage of cells in G0/G1 phase decreased as the percentage of cells in G2/M phase significantly increased in cultures treated with NS-398. NS-398 also induced the apoptosis detected by flow cytometry method. We found that NS-398 treatment significantly downregulated the expression of survivin, cyclinD1 and PTEN in a dose-dependence and time-dependent manner (P<0.001). Conclusion: This study suggests that NS-398 can inhibited the proliferation and induced apoptosis of hepatoma cell, which may be partially fulfilled by downregulation of the survivin and PTEN.更多还原