【摘要】 目的:探讨凋亡抑制蛋白(inhibitorofapoptosisprotein,IAP)家族的新成员Livin及其两种异构体Livinα和Livinβ在非小细胞肺癌(NSCLC)组织中的表达及其与NSCLC临床病理的关系。方法:采用逆转录多聚酶链式反应(RT-PCR)检测NSCLC组织中LivinαmRNA和LivinβmRNA的表达,并用westernblot分析Livin蛋白的表达。结果:45例NSCLC组织中LivinmRNA表达阳性率为71.1%,而在癌旁组织和肺良性瘤样病变低表达(5.7%和6.7%,P<0.01)。LivinmRNA的表达与肺癌患者的病理分型﹑肿瘤分化程度﹑淋巴结转移及TNM分期均无明显相关关系。结论:Livin在非小细胞肺癌组织中特异性地高表达及与肿瘤的发生﹑发展的密切相关性,可成为诊断和治疗的新靶点。更多还原

【Abstract】 Objective: To examine and analyze the expression of two isoforms of Livin (Livinα and Livinβ), a novel inhibitor of apoptosis protein(IAP) family member, in non-small cell lung cancer(NSCLC) and to explore relationship between Livin expression and NSCLC histological types. Methods: Livin mRNA and protein expression levels in NSCLC tissues were detected by reverse transcription polymerase chain reaction (RT-PCR) and western blot assay, respectively. Result: Livin mRNA and protein were expressed in 32 of 45 NSCLC cases (71.1%). Livin mRNA expressed low levels in para-cancerous or benign tumors(5.7%and 6.7%, P<0.01). Livin expression had no significant relationship with clinically pathological characteristics of lung cancer patients. Conclusion: Livin gene may play a role in NSCLC development and increased expression of livin mRNA may serve as a new target for lung cancer diagnosis and treatment.更多还原