【摘要】 目的:探讨检测端粒酶活性在膀胱癌早期诊断中的临床意义。方法:采用TRAP-PCR-ELISA法检测32例膀胱癌患者尿液和膀胱冲洗液中脱落细胞、膀胱癌组织、20例正常膀胱组织及14例非膀胱肿瘤患者尿液脱落细胞中端粒酶活性,并行尿脱落细胞学检查。结果:32例膀胱癌患者尿液脱落细胞、膀胱冲洗液脱落细胞、膀胱癌组织中端粒酶活性阳性率分别为65.6%(21/32)、71.9%(23/32)和84.0%(27/32),20例正常膀胱组织端粒酶活性均为阴性,14例非膀胱肿瘤患者尿液中1例端粒酶活性阳性。端粒酶活性阳性表达与肿瘤的分级、分期之间差异无明显相关性(P>0.05),敏感性明显高于脱落细胞病理学检查。结论:尿液、膀胱冲洗液脱落细胞端粒酶活性测定敏感性较高,可用于膀胱癌的早期诊断。更多还原

【Abstract】 Objective: To investigate the Clinical significance in detection of telomerase activation for the early diagnosis of bladder carcinoma. Methods: TRAP-PCR-ELISA assay was conducted for the presence of telomerase activity in voided urine, bladder washings-exfoliated cells, tissue of bladder cancer and, 20 cases of normal bladder tissue, with 14 cases of non-bladder tumor as control. The urinary exfoliative cytology was conducted. Results: Telomerase activation was detected in voided urine, exfoliated cells and tissue of bladder cancer of the 32 cases. And positive expression rate was 65.6%, 71.9% and 84.0%, respectively. The expression of telomerase activation was negative in 20 cases with normal bladder tissue. One of the 14 cases with non-bladder tumor was positive. The expression of telomerase activation wasn’t related to the grades and phases of bladder carcinoma (P>0.05). Conclusions: The detection of telomerase activation is more sensitive and specific than other assays in bladder carcinoma, and would therefore be a good method of early diagnosis.更多还原