【摘要】 目的探讨针刺延缓衰老的作用机制。方法采用快速老化小鼠SAMP10、SAMR1为模型,运用RT-PCR和地高辛标记的非放射性Northern blot技术,观察8月龄SAMR1对照组、SAMP10对照组、SAMP10针刺组及SAMP10非穴位刺激组全脑、皮层和海马HSP84、HSP86基因表达的变化。结果SAMP10对照组小鼠热休克蛋白HSP84、HSP86在全脑、皮层、海马中的表达下调,针刺后表达上调并趋向于正常组。结论SAMP10小鼠脑衰老与热休克蛋白HSP84、HSP86基因表达异常有关,针刺可以通过调节HSP84、HSP86基因表达增强细胞保护、抑制细胞凋亡、抵抗氧化应激,从而起到延缓衰老的作用。更多还原

【Abstract】 Objective To probe the mechanism of acupuncture for anti-aging. Methods In the senescence accelerated mouse the SAMP10 and the SAMR1, by using RT-PCR and DIG-labeled Northern blot technique, the expression differences of HSP84 and HSP86 genes in whole brain, cortex and hippocampus in the 4 groups,8-month SAMR1 control group, 8-month SAMP10 control group, 8-month SAMP10 acupuncture group and 8-month SAMP10 non-point acupuncture group were investigated. Results In the SAMP10 control group, the expression of HSP84 and HSP86 were down-regulated in the whole brain, the cortex and the hippocampus, and they were up-regulated after acupuncture, tending to the normal group. Conclusion Brain aging of the SAMP10 mouse is related with abnormal expression of HSP84 and HSP86 genes, and acupuncture can strengthen the protection of cells, inhibit apoptosis and anti-oxidative stress through regulating expression of HSP84 and HSP86, hence anti-aging.更多还原