【摘要】 目的:探讨电针预处理对胃黏膜损伤大鼠胃黏膜组织肠三叶因子(intestinaltrefoilfactor,ITF)基因表达的影响及意义。方法:将40只大鼠随机分为空白组、模型组、胃经组、胆经组。空白组不做任何处理,而模型组仅捆缚7d后造模,胃经组、胆经组须先捆缚电针7d后再造模。造模采用水浸束缚法,水浸束缚10h,经相应处理后检测胃黏膜损伤指数(UI),同时取各组大鼠胃黏膜组织,采用RTPCR方法测胃黏膜组织肠三叶因子基因(ITFmRNA)表达。结果:①胃经组、胆经组与模型组的UI比较差异有显著或非常显著性意义(P<0.05或P<0.01),说明造模成功;②电针可提高胃黏膜组织ITFmRNA表达水平,胆经组较模型组ITFmRNA表达增高,胃经组较胆经组ITFmRNA表达非常显著升高(P<0.01)。结论:电针对胃黏膜组织特异性调整作用,与肠三叶因子基因表达差异有关。更多还原

【Abstract】 Objective To investigate effects of electroacupuncture on gastric mucosal intestinal trefoil factor (ITF) gene expression in the rat of gastric mucosal injury induced by stress. Methods Forty rats were randomly divided into 4 groups: blank group, model group, stomach channel group, gallbladder channel group. The normal group did not receive any processing, and the model group were fixed for 7 sessions and both the stomach channel group and the gallbladder channel group received 7 sessions of electro-acupuncture before modeling. The model was made by water restraint stress (WRS) for 10 hours. Index of gastric mucosal injury was detected and then the gastric mucosa tissue in each rat was taken and the expression of ITFmRNA of the tissue was detected by the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Results There was very significant or significant difference in the gastric mucosal injury as the stomach channel group and the gallbladder channel group compared with the model group (P<0.05 or P<0.01); electroacupuncture had a tendency of improving the expression of gastric mucosal ITFmRNA gene; the expression of ITFmRNA in the gallbladder channel group and the stomach channel group was significantly higher than that of the model group (P<0.05,P<0.01). Conclusion The special regulation of electroacupuncture on gastric mucosal tissue is related with the release of epidermal growth factor and the expression of intestinal trefoil factor gene.更多还原