【摘要】 通过在自然界采集、室内平板初筛及平板变色反应复筛得到了20株白腐菌菌株,并对变色系数最小的一株菌株G进行松木片固体接种实验。测定经2种不同浓度无机盐稀释的菌悬液接种松木片3天、5天、7天、9天后的木素、树脂降解情况。结果显示,随着接种时间增加,松木片失重率逐渐增大,Klason木素、酸溶木素和总木素含量逐渐下降,苯-醇抽出物和二氯甲烷抽出物含量也逐渐减少。表面扫描电镜照片表明,接种白腐菌的木片细胞壁上产生了明显的腐朽沟槽等现象;白腐菌菌丝在木片表面的生长呈由少到多、由凌乱到较有规律排列;松木片切片断面显微结构显示菌丝在细胞壁部位及胞间层也开始出现,这说明菌丝开始向细胞内部渗透。更多还原

【Abstract】 Twenty strains were obtained by petri dishes screening and petri dishes color reaction screening.One of the strains G with minimum Mycel/Col value was inoculated onto the Masson Pine chips surfaces.The degradation rates of lignin and pitch were determined after 3 days,5 days,7 days and 9 days respectively. The wood weight loss increased along with the inoculation time increased.The contents of Klason lignin,acid soluble lignin and total lignin decreased during the 3 days to 9 days period of strain inoculation.The contents of dichloromethane (DCM) extractives and alcoholbenzene solubles in wood decreased gradually when the inoculation time continued.The growth of the white-rot fungus strain G and the ultrastructural change of the wood were studied by scanning electron microscope(SEM).The results showed that G not only had great ability to degrade lignin in a short time,but also had a good ability to reproduce the hypha.Besides,the crosssection study of Masson Pine also showed that hypha appeared on the cell walls and the intercellular layers.更多还原